scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agencyscbmt-102Research ArticleНОВЫЕ БИОМЕДИЦИНСКИЕ ИТЕХНОЛОГИИАнализ профиля экспрессии генов NOS2, PDE5A и HIF-1α методом цифровой капельной ПЦРAnalysis of the gene expression profile NOS2, PDE5A and HIF-1α by the digital droplet PCRКаркищенкоВ. Н.KarkischenkoV. N.noemail@neicon.ruДуляМ. С.DulyaM. S.mduliya@gmail.comАгельдиновР. А.AgeldinovR. A.ageldinov@gmail.comПетроваН. В.PetrovaN. V.noemail@neicon.ruФокинЮ. В.FokinYu. V.noemail@neicon.ruХвостовД. В.KhvostovD. V.noemail@neicon.ruГБУН «Научный центр биомедицинских технологий ФМБА России»Россия20182602201903116Copyright © Каркищенко В.Н., Дуля М.С., Агельдинов Р.А., Петрова Н.В., Фокин Ю.В., Хвостов Д.В., 20192019Каркищенко В.Н., Дуля М.С., Агельдинов Р.А., Петрова Н.В., Фокин Ю.В., Хвостов Д.В.Karkischenko V.N., Dulya M.S., Ageldinov R.A., Petrova N.V., Fokin Y.V., Khvostov D.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/102

Исследовано влияние продукта выделения мускусных желез кабарги Moschus moschiferus путем определения уровней экспрессии генов молекул субъединицы 1α гипоксией индуцибельного фактора, индуцибельной изоформы синтазы оксида азота и циклического гуанозинмонофосфата - специфической фосфодиэстеразы типа 5A у экспериментальных животных в условиях стресса, вызванного физической нагрузкой. В работе были исследованы биологические материалы (мозг, сердце и легкие) крыс Wistar, получавших изучаемое вещество и подвергавшихся тесту вынужденного плавания с грузом. Профиль экспрессии генов определялся методом цифровой капельной полимеразной цепной реакции. На фоне введения тестируемых веществ уровни экспрессии изучаемых генов характеризуются ожидаемыми значениями, что демонстрирует ускоренный запуск многофакторных механизмов адаптации организма и усиливает способность тканей переживать неблагоприятные условия при введении активных компонентов мускусных желез кабарги Moschus moschiferus .

The effect of the release product of the musk glands of Moschus moschiferus was determined by determining the expression levels of the molecules of the 1α subunit of the hypoxia inducible factor, inducible nitric oxide synthase and cyclic guanosine monophosphate -specific phosphodiesterase type 5A in experimental animals under stress caused by forced activity. The biological materials (brain, heart and lungs) of Wistar rats that received the substance under study and undergone a forced swim test with a cargo were examined in the work. The gene expression profile was determined by droplet digital polymerase chain reaction. Against the background of the introduction of test substances, the expression levels of the studied are characterized by the expected values, which demonstrate the accelerated start of multifactorial mechanisms of adaptation of the organism and amplifies the ability of tissues to experience unfavorable conditions upon administration of active components of the musk glands of Moschus moschiferus .

кабарга сибирская Moschus moschiferusмускусцифровая капельная полимерная цепная реакциясубъединица 1α гипоксией индуцибельного фактораиндуцибельная синтаза оксида азотациклический гуанозинмонофосфат -специфическая фосфодиэстераза типа 5AэкспрессияSiberian musk Moschus moschiferusdroplet digital polymerase chain reactionsubunit 1α of the hypoxia inducible factorinducible nitric oxide synthasecyclic guanosine monophosphate - specific phosphodiesterase type 5AexpressionReferencesАгаджанян Н.А., Труханов А.И., Шендеров Б.А. Этюды об адаптации и путях сохранения здоровья. - М.: Изд-во «Сирин». - 2002. - 156 с.Агаджанян Н.А., Труханов А.И., Шендеров Б.А. Этюды об адаптации и путях сохранения здоровья. - М.: Изд-во «Сирин». - 2002. - 156 с.Гаркави Л.Х., Квакина Е.Б., Уколова М.А. Адаптационные реакции и резистентность организма. - Ростов н/Д: Изд. РГУ. - 1990. - 224 с.Гаркави Л.Х., Квакина Е.Б., Уколова М.А. Адаптационные реакции и резистентность организма. - Ростов н/Д: Изд. 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Pp. 4180-4184.Lowenstein C.J., Glatt C.S., Bredt D.S., Snyder S.H. Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proceedings of the National Academy of Sciences of the USA. 1992. No. 89(15). Pp. 6711-6715.Lowenstein C.J., Glatt C.S., Bredt D.S., Snyder S.H. Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proceedings of the National Academy of Sciences of the USA. 1992. No. 89(15). Pp. 6711-6715.Nardozza A., Cabrini M.R. Daily use of phosphodiesterase type 5 inhibitors as prevention for recurrent priapism. Rev. Assoc. Med. Bras. 2017. No. 63(8). Pp. 689-692.Nardozza A., Cabrini M.R. Daily use of phosphodiesterase type 5 inhibitors as prevention for recurrent priapism. Rev. Assoc. Med. Bras. 2017. No. 63(8). Pp. 689-692.Pacher P., Beckman J.S., Liaudet L. Nitric Oxide and Peroxynitrite: in Health and disease. Physiological Reviews. 2007. No. 87(1). Pp. 315-424.Pacher P., Beckman J.S., Liaudet L. Nitric Oxide and Peroxynitrite: in Health and disease. Physiological Reviews. 2007. No. 87(1). Pp. 315-424.Porasuphatana S., Weaver J., Rosen G.M. Inducible nitric oxide synthase catalyzes ethanol oxidation to alpha-hydroxyethyl radical and acetaldehyde. Toxicology. 2006. No. 223. Pp. 167-174.Porasuphatana S., Weaver J., Rosen G.M. Inducible nitric oxide synthase catalyzes ethanol oxidation to alpha-hydroxyethyl radical and acetaldehyde. Toxicology. 2006. No. 223. Pp. 167-174.Semenza G.L., Agani F., Iyer N., Kotch L., Laughner E., Leung S., Yu A. Regulation of cardiovascular development and physiology by hypoxia-inducible factor 1. Annals of the New York Academy of Sciences. 1999. No. 874. Pp. 262-268.Semenza G.L., Agani F., Iyer N., Kotch L., Laughner E., Leung S., Yu A. Regulation of cardiovascular development and physiology by hypoxia-inducible factor 1. Annals of the New York Academy of Sciences. 1999. No. 874. Pp. 262-268.Uzunov P., Weiss B. Separation of multiple molecular forms of cyclic adenosine-3', 5’-monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis. Biochim. Biophys. Acta. 1972. No. 284(1). Pp. 220-226.Uzunov P., Weiss B. Separation of multiple molecular forms of cyclic adenosine-3', 5’-monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis. Biochim. Biophys. Acta. 1972. No. 284(1). Pp. 220-226.Weiss B. Differential activation and inhibition of the multiple forms of cyclic nucleotide phosphodiesterase. Adv. Cyclic Nucleotide Res. 1975. Vol. 5. Pp. 195-211.Weiss B. Differential activation and inhibition of the multiple forms of cyclic nucleotide phosphodiesterase. Adv. Cyclic Nucleotide Res. 1975. Vol. 5. Pp. 195-211.https://www.ncbi.nlm.nih.gov/gene/24599https://www.ncbi.nlm.nih.gov/gene/24599https://www.ncbi.nlm.nih.gov/gene/29560https://www.ncbi.nlm.nih.gov/gene/29560https://www.ncbi.nlm.nih.gov/gene/171115https://www.ncbi.nlm.nih.gov/gene/171115

The authors declare that there are no conflicts of interest present.