scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agencyscbmt-1166Research ArticleКЛИНИЧЕСКАЯ ФАРМАКОЛОГИЯПрименение биологической модели для оценки кишечной проницаемости in vitro – монослоя эпителиальных клеток Сасо-2Application of biological model for assessment of intestinal permeability of in vitro – a monolayer of epitelialny Caco-2 cellsШохинИ. Е.ShohinI. E.noemail@neicon.ruКулиничЮ. И.KulinichJ. I.noemail@neicon.ruРаменскаяГ. В.RamenskayaG. V.noemail@neicon.ruКукесВ. Г.KukesV. G.elmed@yandex.ruГОУ ВПО Первый МГМУ им. И.М. Сеченова, Минздравсоцразвития РФ, Москва; ФГБУ «НЦ ЭСМП», Минздравсоцразвития РФ, МоскваRussian FederationГОУ ВПО Первый МГМУ им. И.М. Сеченова, Минздравсоцразвития РФ, Москва; ФГБУ «НЦ ЭСМП», Минздравсоцразвития РФ, Москва; Филиал «Клиническая Фармакология» НЦБМТ РАМН, МоскваRussian Federation201218022020139197Copyright © Шохин И.Е., Кулинич Ю.И., Раменская Г.В., Кукес В.Г., 20202020Шохин И.Е., Кулинич Ю.И., Раменская Г.В., Кукес В.Г.Shohin I.E., Kulinich J.I., Ramenskaya G.V., Kukes V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1166

Статья посвящена применению монокультуры клеток аденокарциномы толстого кишечника – Caco-2 для оценки проницаемости лекарственных веществ (ЛВ) in vitro. Описаны основные преимущества ис- пользования данной биологической модели. Приведены общие принципы, подходы и методология опре- деления проницаемости на культуре клеток Сасо-2. Показана корреляция между значениями кишечной проницаемостью, определенной в условиях in vivo и кажущимися коэффициентами кишечной проница- емости in vitro.

Paper is devoted to the application of epithelial monolayer of human colon adenocarcinoma cells – Caco-2 for in vitro permeability evaluation of drugs. Main advantages of this biological model are described. General principles, approaches and methodology of Caco-2 permeability assay are defined. Correlation between human intestinal permeability in vitro and apparent permeability coefficient in vitro is indicated.

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The authors declare that there are no conflicts of interest present.