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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">scbmt</journal-id><journal-title-group><journal-title xml:lang="ru">БИОМЕДИЦИНА</journal-title><trans-title-group xml:lang="en"><trans-title>Journal Biomed</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2074-5982</issn><issn pub-type="epub">2713-0428</issn><publisher><publisher-name>Scientific center of biomedical technologies of Federal Medical and Biological Agency</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33647/2074-5982-16-3-48-53</article-id><article-id custom-type="elpub" pub-id-type="custom">scbmt-1196</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МЕТОДЫ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>METHODS OF BIOMEDICAL RESEARCHES</subject></subj-group></article-categories><title-group><article-title>Оптимизация протокола гормональной обработки мышей c целью вызывания суперовуляции</article-title><trans-title-group xml:lang="en"><trans-title>Optimizing the Hormonal Mice Treatment Protocol to Induce Superovulation</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Савченко</surname><given-names>Е. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Savchenko</surname><given-names>E. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Савченко Елена Сергеевна</p><p>143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1</p></bio><bio xml:lang="en"><p>Elena S. Savchenko</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1</p></bio><email xlink:type="simple">savelaine@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Огнева</surname><given-names>Н. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Ogneva</surname><given-names>N. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Огнева Настасья Сергеевна</p><p>143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1</p></bio><bio xml:lang="en"><p>Nastasya S. Ogneva</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1</p></bio><email xlink:type="simple">ognevanastya@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Максименко</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Maksimenko</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Максименко Сергей Васильевич, к.б.н.</p><p>143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1</p></bio><bio xml:lang="en"><p>Sergey V. Maksimenko, Cand. Sci. (Biol.)</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1</p></bio><email xlink:type="simple">vx136@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Скрипкина</surname><given-names>М. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Skripkina</surname><given-names>M. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Скрипкина Мария Михайловна</p><p>143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1</p></bio><bio xml:lang="en"><p>Maria M. Skripkina</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1</p></bio><email xlink:type="simple">skripkina.fmba@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Петрова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Petrova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Петрова Наталья Владимировна</p><p>143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1</p></bio><bio xml:lang="en"><p>Natalya V. Petrova</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1</p></bio><email xlink:type="simple">m-sklad@ya.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБУН «Научный центр биомедицинских технологий Федерального медико-биологического агентства России»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Scientiﬁc Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>02</day><month>09</month><year>2020</year></pub-date><volume>16</volume><issue>3</issue><fpage>48</fpage><lpage>53</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Савченко Е.С., Огнева Н.С., Максименко С.В., Скрипкина М.М., Петрова Н.В., 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">Савченко Е.С., Огнева Н.С., Максименко С.В., Скрипкина М.М., Петрова Н.В.</copyright-holder><copyright-holder xml:lang="en">Savchenko E.S., Ogneva N.S., Maksimenko S.V., Skripkina M.M., Petrova N.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.scbmt.ru/jour/article/view/1196">https://journal.scbmt.ru/jour/article/view/1196</self-uri><abstract><p>Гормональная обработка мышей с использованием гонадотропина сыворотки жеребой кобылы (ГСЖК) и хорионического гонадотропина человека (ХГч) считается «золотым стандартом» в процедуре вызывания суперовуляции. Несмотря на применение одних и тех же доз, эффект от использования препаратов разных производителей может отличаться. Так, нами было показано, что применение препаратов ГСЖК Синхростим 500 («Ceva Sante Animale», Франция) и Сергон («Биовета», Чехия) позволяет получить с одной особи ~25 эмбрионов при дозировке гормона 5 МЕ, тогда как в случае Фоллимага («Мосагроген», Россия) рабочая дозировка не была найдена. Сопоставимый с Синхростимом 500 и Сергоном результат был получен в случае применения комбинированного препарата Менопур («Ферринг», Германия). Исследования влияния возраста мышей на гормональный ответ организма показали, что наиболее эффективным является использование мышей в возрасте 3–4 недели, когда при минимальной лекарственной нагрузке мы получаем достаточное количество жизнеспособных эмбрионов.</p></abstract><trans-abstract xml:lang="en"><p>Mice treatment with pregnant mare gonadotropin serum (PMGS) in combination with human chorionic gonadotropin (hCG) is considered to be the “golden standard” of the induced superovulation procedure. The effect of administering the same doses may differ depending on the manufacturer of the preparation. According to our results, the use of PMGS produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female. At the same time, the working dose of Follimagum (Mosagrogen, Russia) was not determined. The result comparable with that of Synchrostim 500 and Sergon was achieved using a composite drug Menopur (Ferring, Germany). The study of the inﬂuence of mice age on the hormonal response showed 3–4 weeks to be the most productive age, when the maximal number of viable embryos was obtained under a minimal external invasion.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>гонадотропин сыворотки жеребой кобылы</kwd><kwd>хорионический гонадотропин человека</kwd><kwd>суперовуляция</kwd><kwd>яйцеклетки</kwd><kwd>трансгенез</kwd><kwd>мыши</kwd><kwd>эмбрионы</kwd></kwd-group><kwd-group xml:lang="en"><kwd>pregnant mare gonadotropin serum</kwd><kwd>human chorionic gonadotropin</kwd><kwd>superovulation</kwd><kwd>eggs</kwd><kwd>transgenesis</kwd><kwd>mice</kwd><kwd>embryos</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Behringer R., Gertsenstein M., Nagy K.V., Nagy A. 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