scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-17-2-46-57scbmt-1270Research ArticleМЕТОДЫ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙMETHODS OF BIOMEDICAL RESEARCHESДинамика биохимических и цитологических показателей крови крыс при моделировании хронической алиментарной метионин-обусловленной гомоцистеинемииDynamics of Biochemical and Cytological Parameters of Rat Blood in Simulated Chronic Alimentary Methionine-Induced HomocysteinemiaПазиненкоК. А.Pazinenko K. A.

Пазиненко Ксения Андреевна

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Kseniya A. Pazinenko 

426056,  Izhevsk, Kommunarov Str., 281 

ЧучковаН. Н.ChuchkovaN. N.

Чучкова Наталья Николаевна*, д.м.н., проф.

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Natalya N. Chuchkova, Dr. Sci. (Med.), Prof.

426056,  Izhevsk, Kommunarov Str., 281 

mig05@inbox.ruСметанинаМ. В.SmetaninaM. V.

Сметанина Марина Викторовна, к.м.н.

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Marina V. Smetanina, Cand. Sci. (Med.)

426056,  Izhevsk, Kommunarov Str., 281 

ПазиненкоО. А.PazinenkoO. A.

Пазиненко Олег Алексеевич

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Oleg A. Pazinenko

426056,  Izhevsk, Kommunarov Str., 281 

ПантелеевК. Э.PanteleevK. E.

Пантелеев Кирилл Эдуардович

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Kirill E. Panteleev

426056,  Izhevsk, Kommunarov Str., 281 

ИвановГ. В.IvanovG. V.

Иванов Григорий Вадимович

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Grigory V. Ivanov

426056,  Izhevsk, Kommunarov Str., 281 

КормилинаН. В.KormilinaN. V.

Кормилина Наталья Владимировна, доц.

426056, Российская Федерация, Ижевск, ул. Коммунаров, 281

Natalya V. Kormilina, Assoc. Prof.

426056,  Izhevsk, Kommunarov Str., 281 

ФГБОУ ВО «Ижевская государственная медицинская академия» Минздрава РоссииРоссияIzhevsk State Medical Academy of the Ministry of Health care of RussiaRussian Federation2021250620211724657Copyright © Пазиненко К.А., Чучкова Н.Н., Сметанина М.В., Пазиненко О.А., Пантелеев К.Э., Иванов Г.В., Кормилина Н.В., 20212021Пазиненко К.А., Чучкова Н.Н., Сметанина М.В., Пазиненко О.А., Пантелеев К.Э., Иванов Г.В., Кормилина Н.В.Pazinenko  K.A., Chuchkova N.N., Smetanina M.V., Pazinenko O.A., Panteleev K.E., Ivanov G.V., Kormilina N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1270

В условиях хронической нагрузки метионином (ежедневное добавление аминокислоты в пищу (0,15 г/100 г) и воду (1% р-р)) в течение 2–12 недель наблюдали динамику показателей печеночных тестов (аланинаминотрансферазы, аспартатаминотрансферазы, щелочной фосфатазы, билирубина), воспалительных изменений в крови, липидного спектра. Установлено, что метиониновая нагрузка (МН) с 4-й недели приводит к умеренной гипергомоцистеинемии (28,9±2,65 против 8,5±0,6 мкмоль/л в контроле), повышению ферментов печени (АсАТ — в 1,73, АлАТ — 1,5 раза, р<0,05) и билирубина (на 62,25%), что указывает на сформированную гепатопатию, которая в дальнейшем (12 недель МН) усугубляется нарушением экскреторной функции печени и холестазом (увеличение щелочной фосфатазы в 1,65, билирубина — в 3,31 раза, р<0,05).

Under the conditions of a chronic methionine diet (daily addition of amino acids to food (0.15 g/100 g) and water (1% solution)) during 2–12 weeks, the dynamics of liver tests, infl ammatory changes in the blood and blood lipids was monitored. It was found that a methionine diet (MD) leads, starting from 4 weeks of MD, to medium hyperhomocysteinemia, an increase in liver enzymes (AsAT – 1.73, AlAT – 1.5 times, p<0.05) and bilirubin (by 62.25%), which indicates the formed hepatopathy. Further (12 weeks of MD), the condition is aggravated by an abnormality of excretory liver function and the development of cholestasis (an increase in alkaline phosphatase by 1.65, bilirubin – by 3.31 times, p<0.05).

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The authors declare that there are no conflicts of interest present.