scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-18-1-32-55scbmt-1364Research ArticleДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ В БИОМЕДИЦИНЕNON-CLINICAL RESEARCH IN BIOMEDICINEЦентральные механизмы липосомированных форм ацетилхолина и инсулина посредством анализа когнитивных, психоэмоциональных и поведенческих параметров крысCentral Mechanisms of Liposomated Forms of Acetylcholin and Insulin Revealed by an Analysis of Cognitive, Psycho-Emotional and Behavioral Parameters of RatsКаркищенкоВ. Н.KarkischenkoV. N.

Каркищенко Владислав Николаевич, д.м.н., проф.

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Vladislav N. Karkischenko, Dr. Sci. (Med.), Prof.

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

scbmt@yandex.ruФокинЮ. В.FokinYu. V.

Фокин Юрий Владимирович, к.б.н.

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Yuriy V. Fokin, Cand. Sci. (Biol.)

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

fokin@scbmt.ruЛюблинскийС. Л.LyublinskiyS. L.

Люблинский Станислав Людвигович, к.б.н.

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Stanislav L. Lyublinskiy, Cand. Sci. (Biol.)

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

mobitek-m@mail.ruПомыткинИ. А.PomytkinI. A.

Помыткин Игорь Анатольевич, к.х.н.

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Igor A. Pomytkin, Cand. Sci. (Chem.)

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

ipomytkin@mail.ruАлимкинаО. В.AlimkinaO. V.

Алимкина Оксана Владимировна

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Oksana V. Alimkina

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

alimkina@scbmt.ruТабояковаЛ. А.TaboyakovaL. A.

Табоякова Лидия Александровна

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Lidiya A. Taboyakova

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

lida-vet@mail.ruКапцовА. В.KaptsovA. V.

Капцов Александр Владимирович

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Alexander V. Kaptsov

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

a_v_kaptsov@mail.ruБорисоваМ. М.BorisovaM. M.

Борисова Мария Михайловна

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Mariya M. Borisova

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

borisova_mm@mail.ruКаркищенкоН. Н.KarkischenkoN. N.

Каркищенко Николай Николаевич, д.м.н., проф., акад. РАРАН, чл.-корр. РАН

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Nikolay N. Karkischenko, Dr. Sci. (Med.), Prof., Academician of the Russian Academy of Rocket and Artillery Sciences, Corresponding Member of the Russian Academy of Sciences

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

niknik2808@yandex.ruФГБУН «Научный центр биомедицинских технологий ФМБА России»РоссияScientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of RussiaRussian Federation2022030420221813255Copyright © Каркищенко В.Н., Фокин Ю.В., Люблинский С.Л., Помыткин И.А., Алимкина О.В., Табоякова Л.А., Капцов А.В., Борисова М.М., Каркищенко Н.Н., 20222022Каркищенко В.Н., Фокин Ю.В., Люблинский С.Л., Помыткин И.А., Алимкина О.В., Табоякова Л.А., Капцов А.В., Борисова М.М., Каркищенко Н.Н.Karkischenko V.N., Fokin Y.V., Lyublinskiy S.L., Pomytkin I.A., Alimkina O.V., Taboyakova L.A., Kaptsov A.V., Borisova M.M., Karkischenko N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1364

Изучены центральные механизмы липосомированных форм ацетилхолина и инсулина при трансмукозальном введении крысам. Анализ параметров ультразвуковой вокализации, свободного поведения и когнитивных функций показал непосредственное влияние тестируемых веществ на основные  механизмы высшей нервной деятельности. Посредством комплексного биомедицинского тестирования установлены и подтверждены анксиолитические признаки с седативным компонентом,  обеспечивающие улучшение консолидации памяти и умственных способностей. Наиболее выраженный эффект по анализу ультразвуковой вокализации обнаруживается для инсулина, а наиболее  информативные этологические параметры крыс по анализу антидепрессивных свойств в лабиринте  установлены на фоне применения ацетилхолина. Курсовое применение в течение 7-ми дней липосомированного инсулина повышает когнитивные способности животных более чем в два раза, ацетилхолина — более чем в четыре раза, что отражает высокочастотные β- и γ-ритмы (выше 20 Гц)  гиппокампа, связанные с активностью вставочных нейронов и пирамидных клеток головного мозга.  Инновационная таргетная доставка лекарственных веществ на основе нейромедиаторов и гормонов  оказывает убедительное влияние ацетилхолина и инсулина на холинергическую и ГАМК-ерическую  системы, а также позволяет моделировать и изучать механизмы и способы лечения нейропатий.

Central mechanisms of the liposomal forms of acetylcholine and insulin were studied during their  transmucosal administration to rats. An analysis of the parameters of ultrasonic vocalization, free behavior  and cognitive functions showed a direct effect of the tested substances on the main mechanisms of higher  nervous activity. By means of complex biomedical testing, anxiolytic signs with a sedative component  were established and confirmed, providing an improvement in the consolidation of memory and mental  abilities. The most pronounced effect in the analysis of ultrasonic vocalization was observed for insulin,  while the most informative ethological parameters of rats in the analysis of antidepressant properties in the  maze test were established for acetylcholine. The administration of liposomal insulin and acetylcholine  for 7 days increases the cognitive abilities of animals by more than two times and four times, respectively.  This reflects high-frequency β- and γ-rhythms (above 20 Hz) of the hippocampus associated with the  activity of intercalary neurons and pyramidal brain cells. Innovative targeted delivery of the drugs based  on neurotransmitters and hormones has a convincing effect of acetylcholine and insulin on the cholinergic  and GABAeric systems. This also facilitates modeling and studying the mechanisms and methods  of treating neuropathies.

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The authors declare that there are no conflicts of interest present.