scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-18-4-10-23scbmt-1427Research ArticleГЕНЕТИКА И ЭПИГЕНЕТИКА ЖИВОТНЫХ-БИОМОДЕЛЕЙGENETICS AND EPIGENETICS OF ANIMALS-BIOMODELSЭмбриологические аспекты создания новой гуманизированной трансгенной линии мышей с интегрированным геном человека HLA-A*02:01:01:01Embryological Aspects of Creating a New Humanized Transgenic Mouse Line with an Integrated Human Gene HLA A*02:01:01:01СавченкоЕ. С.SavchenkoE. S.

Савченко Елена Сергеевна

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Elena S. Savchenko

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

savelaine@gmail.comОгневаН. С.OgnevaN. S.

Огнева Настасья Сергеевна

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Nastasya S. Ogneva

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

ognevanastya@mail.ruКаркищенкоН. Н.KarkischenkoN. N.

Каркищенко Николай Николаевич, д.м.н., проф., чл.-корр. РАН, акад. РАРАН

143442, Московская обл., Красногорский р-н, п. Светлые горы, 1

Nikolay N. Karkischenko, Dr. Sci. (Med.), Prof., Corr. Member of the Russian Academy of Sciences, Acad. of the Russian Academy of Rocket and Artillery Sciences

143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1

niknik2808@yandex.ruФГБУН «Научный центр биомедицинских технологий ФМБА России»РоссияScientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of RussiaRussian Federation2022111220221841023Copyright © Савченко Е.С., Огнева Н.С., Каркищенко Н.Н., 20222022Савченко Е.С., Огнева Н.С., Каркищенко Н.Н.Savchenko E.S., Ogneva N.S., Karkischenko N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1427

Накопление научных данных в области фармакогенетики требует создания адекватных релевантных биомоделей, отражающих иммуногенетические особенности отдельных групп населения. Нами были получены родоначальники новой гуманизированной трансгенной линии мышей, несущих ген HLA-A*02:01:01:01 человека, характерный для населения России. Для создания новой биомодели был использован метод инъекции генно-инженерной конструкции в пронуклеусы зигот с последующим культивированием в СО2-инкубаторе в течение ночи и переносом потенциально модифицированных эмбрионов на стадии двух бластомеров псевдобеременным самкам-реципиентам. Всего был получен и проанализирован 91 живой потомок, из которых 18 несли целевую модификацию генома. Полученные трансгенные животные были использованы для выведения новой линии животных-биомоделей, несущих ген HLA-A*02:01:01:01 человека.

The accumulation of scientific data in the field of pharmacogenetics requires the creation of adequate biomodels to reflect the immunogenetic characteristics of different population groups. We have obtained the ancestors of a new humanized transgenic mouse line carrying the human HLA-A*02:01:01:01 gene, which is characteristic of the Russian population. The new biomodels was created using the pronuclei microinjection method of a linearized fragment of genetically engineered DNA construct into zygotes, followed by overnight cultivation in CO2 incubator and transfer of potentially modified embryos at the stage of two 4.0 blastomere to pseudopregnant foster females. A total of 91 living offspring were obtained and analyzed, with 18 pups carrying the target genome modification. The resulting transgenic animals were used to create a new line of mouse biomodels carrying the human HLA-A*02:01:01:01 gene.

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The authors declare that there are no conflicts of interest present.