scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-18-4-112-122scbmt-1436Research ArticleРЕЛЕВАНТНОЕ И АЛЬТЕРНАТИВНОЕ БИОМОДЕЛИРОВАНИЕRELEVANT AND ALTERNATIVE BIOMODELLINGНарушение экспрессии мРНК мю- и каппа-опиоидных рецепторов в среднем мозге крыс с пренатальной алкогольной интоксикациейImpaired Expression of Mu- and Kappa-Opioid Receptor mRNA in the Midbrain of Rats with Prenatal Alcohol IntoxicationКоханВ. С.KokhanV. S.

Кохан Виктор Сергеевич, к.б.н.

119002, Москва, Малый Могильцевский пер., 3

Viktor S. Kokhan, Cand. Sci. (Biol.)

119002, Moscow, Maliy Mogiltsevskiy Lane, 3

viktor_kohan@hotmail.comАнохинП. К.AnokhinP. K.

Анохин Пётр Константинович, к.б.н.

119002, Москва, Малый Могильцевский пер., 3

Petr K. Anokhin, Cand. Sci. (Biol.)

119002, Moscow, Maliy Mogiltsevskiy Lane, 3

petranokhin@mail.ruПахловаЕ. П.PakhlovaE. P.

Пахлова Екатерина Павловна

119234, Москва, ул. Ленинские горы, 1/12

Ekaterina P. Pakhlova

119234, Moscow, Leninskiye Gory Str., 1/12

kpakhlova@gmail.comСарычеваН. Ю.SarychevaN. Yu.

Сарычева Наталия Юрьевна, к.б.н.

119234, Москва, ул. Ленинские горы, 1/12

Natalia Yu. Sarycheva, Cand. Sci. (Biol.)

119234, Moscow, Leninskiye Gory Str., 1/12

nsarychava@gmail.comШамакинаИ. Ю.ShamakinaI. Yu.

Шамакина Инна Юрьевна, к.б.н.

119002, Москва, Малый Могильцевский пер., 3

Inna Yu. Shamakina, Cand. Sci. (Biol.)

119002, Moscow, Maliy Mogiltsevskiy Lane, 3

shamakina@yahoo.comНациональный научный центр наркологии — филиал ФГБУ «Национальный медицинский исследовательский центр психиатрии и наркологии им. В.П. Сербского» Минздрава РоссииРоссияNational Scientific Center for Narcology — Branch of the V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology of the Ministry of Health Care of RussiaRussian FederationФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова»РоссияLomonosov Moscow State UniversityRussian Federation202212122022184112122Copyright © Кохан В.С., Анохин П.К., Пахлова Е.П., Сарычева Н.Ю., Шамакина И.Ю., 20222022Кохан В.С., Анохин П.К., Пахлова Е.П., Сарычева Н.Ю., Шамакина И.Ю.Kokhan V.S., Anokhin P.K., Pakhlova E.P., Sarycheva N.Y., Shamakina I.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1436

Нарушение эпигенетического репрограммирования под действием экзогенных факторов в пренатальном периоде отражается на развитии и формировании фенотипа взрослого организма. Один из таких факторов – алкоголь, воздействие которого в период пренатального онтогенеза может приводить к высокому риску злоупотребления алкоголем в будущем. Механизмы, лежащие в основе этого эффекта, в настоящее время мало изучены. Мы предполагаем, что изменение «подкрепляющего» действия алкоголя в результате пренатальной алкоголизации может быть связано с нарушением транскрипционной активности генов опиоидной системы мозга. В работе проведён сравнительный анализ добровольного потребления алкоголя и уровня экспрессии мРНК μ- (MOP) и κ-опиоидных (KOP) рецепторов в мезолимбических областях мозга взрослых крыс-самцов Wistar (PND60) – потомков самок, потреблявших 10%-ный р-р этанола со 2-го по 21-й дни беременности, и контрольных животных. Пренатально алкоголизированные крысы отличались от контрольных высоким предпочтением алкоголя и низким уровнем экспрессии мРНК MOР и KOР в среднем мозге. Выявленные нарушения могут лежать в основе дисфункции опиатной системы и, как следствие, высокого риска формирования аддиктивного поведения во взрослом возрасте.

Disruption of normal epigenetic reprogramming during the prenatal period under the influence of exogenous factors affects fetus development and adult phenotype formation. The mechanisms through which determinants, such as maternal alcohol intake, contribute to the formation of an alcohol-vulnerable phenotype later in life still remain unclear. In this paper, we suggest that alteration in the reinforcing properties of ethanol in prenatally alcohol-exposed subjects may be associated with transcriptional dysregulation of the brain opioid receptor genes. We compared voluntary alcohol intake and levels of mRNA coding for μ- (MOP) and κ-opioid (KOP) receptors in the mesolimbic areas of adult male offspring of the female Wistar rats having received 10% ethanol as the only source of liquid throughout pregnancy or water (control). We found that prenatally alcohol exposed rats had higher alcohol preference on PND60 (free-choice paradigm) and lower mRNA expression for both MOP and KOP in the midbrain compared to the control. This suggests a potential link between prenatal alcohol, dysfunction of the brain opiate system and adult vulnerability for alcohol use disorder.

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The authors declare that there are no conflicts of interest present.