scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-19-1-34-46scbmt-1455Research ArticleМЕТОДЫ И ТЕХНОЛОГИИ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙMETHODS AND TECHNOLOGIES OF BIOMEDICAL RESEARCHАнализ основных мировых трендов в объективизации протоколов поведенческого тестирования лабораторных животных с патологией головного мозгаAnalysis of Main World Trends in Objectivization of Protocols for Behavioral Testing of Laboratory Animals with Brain PathologyСалминаА. Б.SalminaA. B.

Салмина Алла Борисовна, д.м.н., проф.

660022, Российская Федерация, Красноярск, ул. Партизана Железняка, 1; 105064,  Москва, ул. Воронцово поле, 14

Alla B. Salmina, Dr. Sci. (Med.), Prof.

660022,  Krasnoyarsk, Partizana Zheleznyaka Str., 1; 105064,  Moscow, Vorontsovo Pole Str., 14

ГоринаЯ. В.GorinaYa. V.

Горина Яна Валерьевна, к.фарм.н., доц

660022, Российская Федерация, Красноярск, ул. Партизана Железняка, 1

Yana V. Gorina*, Cand. Sci. (Pharm.), Assoc. Prof.

660022,  Krasnoyarsk, Partizana Zheleznyaka Str., 1

yana_20@bk.ruБольшаковаА. В.BolshakovaA. V.

Большакова Анастасия Викторовна, к.б.н.

194021, Санкт-Петербург, ул. Хлопина, 11

Anastasia V. Bolshakova, Cand. Sci. (Biol.)

194021, St. Petersburg, Khlopina Str., 11

ВласоваО. Л.VlasovaO. L.

Власова Ольга Леонардовна, д.ф-м.н., доц.

194021, Санкт-Петербург, ул. Хлопина, 11

Olga L. Vlasova, Dr. Sci. (Phys.-Math.), Assoc. Prof.

194021, St. Petersburg, Khlopina Str., 11

НИИ молекулярной медицины и патобиохимии, ФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого» Минздрава России; Институт мозга, ФГБНУ «Научный центр неврологии»РоссияResearch Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Health Care of Russia; Brain Institute, Research Center of NeurologyRussian FederationНИИ молекулярной медицины и патобиохимии, ФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого» Минздрава РоссииРоссияResearch Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Health Care of RussiaRussian FederationФГАОУ ВО «Санкт-Петербургский политехнический университет Петра Великого»РоссияSt. Petersburg Polytechnic University of Peter the GreatRussian Federation2023070420231913446Copyright © Салмина А.Б., Горина Я.В., Большакова А.В., Власова О.Л., 20232023Салмина А.Б., Горина Я.В., Большакова А.В., Власова О.Л.Salmina A.B., Gorina Y.V., Bolshakova A.V., Vlasova O.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1455

Поведенческое фенотипирование грызунов с моделями нейродегенерации привлекает большое внимание учёных в течение последних трёх десятилетий. Тем не менее, по-прежнему существуют определённые сложности в понимании изменчивости поведения, вызванной генетическими, экологическими и биологическими факторами, вмешательством человека и плохо стандартизированными экспериментальными протоколами, что может отрицательно отразиться на интерпретации полученных результатов. В этой статье мы представляем факторы, оказывающие негативное влияние на качество выполнения поведенческого тестирования лабораторных животных, современные подходы по их преодолению, а также новые технологии, такие как визуализация активности нейронов с помощью ионно-зависимых флуоресцентных индикаторов (оптогенетика), которые расширяют границы изучения нейронных сетей, ответственных за поведение, путём оценки функции нейронов как на клеточном, так и на популяционном уровнях, что, в итоге, позволит повысить надёжность полученных результатов и даст возможность по-новому взглянуть на этологические парадигмы конкретной трансгенной мышиной модели.

Behavioral phenotyping of rodents using neurodegeneration models has received much research attention over the past three decades. However, some difficulties still exist in understanding the variability of behavior caused by genetic, environmental, and biological factors, human intervention and poorly standardized experimental protocols, which can negatively affect the interpretation of the results obtained. In this article, we discuss factors that have a negative impact on the performance of behavioral testing of laboratory animals, modern approaches to overcome them, as well as new technologies, such as visualization of neuronal activity using ion-dependent fluorescent indicators (optogenetics), which expand the boundaries of the study of neuronal networks responsible for behavior by evaluating neuronal function at both the cellular and population levels. Ultimately, this will increase the reliability of the results obtained and provide an opportunity to take a fresh look at the ethological paradigms of a particular transgenic mouse model.

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The authors declare that there are no conflicts of interest present.