References

scbmt

БИОМЕДИЦИНА

Journal Biomed

2074-59822713-0428

Scientific center of biomedical technologies of Federal Medical and Biological Agency

10.33647/2074-5982-15-4-12-33

scbmt-148

Research Article

НОВЫЕ БИОМЕДИЦИНСКИЕ ИТЕХНОЛОГИИ

ТРАНСГЕННЫЕ И НОКАУТНЫЕ КРОЛИКИ В БИОМЕДИЦИНЕ И ГЕНОТЕРАПИИ. CRISPR/CAS9-ТЕХНОЛОГИИ (ОБЗОР)

RABBIT BIOMODELS OF HUMAN DISEASES DEVELOPED USING NEW GENOMIC TECHNOLOGIES. CRISPR/CAS9 (REVIEW)

Колоскова

Е. М.

Koloskova

E. M.

к.б.н.,

249013, Калужская обл., Боровск, пос. Институт

Cand. Sci. (Biol.), All-Russian Research Institute of Physiology,

249013, Kaluga region,Borovsk, Institut village

heleko3@yandex.ru

Каркищенко

В. Н.

Karkischenko

V. N.

д.м.н., проф.,

143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1

Dr. Sci. (Med.), Prof.,

143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1

scbmt@yandex.ru

Езерский

В. А.

Yezersky

V. A.

249013, Калужская обл., Боровск, пос. Институт

All-Russian Research Institute of Physiology,

249013, Kaluga region,Borovsk, Institut village

ez.vadim@yandex.ru

Петрова

Н. В.

Petrova

N. V.

143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1

143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1

m-sklad@yandex.ru

Максименко

С. В.

Maksimenko

S. V.

к.б.н.,

143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1

Cand. Sci. (Biol.),

143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1

vx136@rambler.ru

Матвеенко

Е. Л.

Matveyenko

E. L.

к.э.н., доц.,

143442, Московская обл., Красногорский р-н, п. Светлые горы, владение 1

Cand. Sci. (Econ.), Assoc. Prof.,

143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1

matveyenkoel@mail.ru

Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных — филиал ФГБНУ «Федеральный научный центр животноводства — ВИЖ им. акад. Л.К. Эрнста»РоссияAll-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — branch of the Federal Scientific Center of Animal Husbandry — The All-Russian Institute of Animal Husbandry named after academician L.K. ErnstRussian Federation

ФГБУН «Научный центр биомедицинских технологий Федерального медико-биологического агентства России»РоссияScientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of RussiaRussian Federation

2019

29112019

041233

2019

Колоскова Е.М., Каркищенко В.Н., Езерский В.А., Петрова Н.В., Максименко С.В., Матвеенко Е.Л.

Koloskova E.M., Karkischenko V.N., Yezersky V.A., Petrova N.V., Maksimenko S.V., Matveyenko E.L.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://journal.scbmt.ru/jour/article/view/148

С появлением эндонуклеазных методов редактирования генома, особенно CRISPR/Cas9, стало возможным методом микроинъекции зигот с высокой эффективностью получать животных — как мышей, так и особенно генно-модифицированных кроликов — для различных целей. В настоящей работе рассматриваются современные мировые достижения по созданию кроликов — биомоделей заболеваний человека с использованием технологий геномного редактирования, явления мозаицизма. Делается заключение о целесообразности получения генетически модифицированных кроликов для биомедицинских исследований и биомоделирования.

With the advent of endonuclease methods of genome editing, particularly CRISPR/Cas9, it has become possible to obtain genetically modified rabbits by microinjection of zygotes. These highly effective human disease models can be used for various purposes. The present review aims to consider modern achievements in the creation of rabbit biomodels of human diseases using the technologies of genetic editing. It is concluded that Russian laboratories should intensify research in the development of genetically modified rabbits that can be used for various biomedical studies and biomodelling.

редактирование генома кроликовтрансгенознокаутCRISPR/Cas9-технологиимозаицизмбиомодели для генной терапии и биомедицины

rabbit genome editingtransgenosisknockoutCRISPR/Cas9 technologiesmosaicismbiomodels for gene therapy and biomedicine

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The authors declare that there are no conflicts of interest present.