scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-20-4-70-77scbmt-1674Research ArticleДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ В БИОМЕДИЦИНЕNON-CLINICAL RESEARCH IN BIOMEDICINEХарактеристика стромального компонента печени после введения аскорбиновой кислоты в модели лучевого гепатитаCharacteristics of the Fibrous Component of the Liver after Administration of Ascorbic Acid in a Radiation Hepatitis ModelДемяшкинГ. А.DemyashkinG. А.

Демяшкин Григорий Александрович, д.м.н.

249036, Российская Федерация, Московская обл., Обнинск, ул. Королева, 4; 119048, Москва, ул. Трубецкая, 8, стр. 2

Grigory А. Demyashkin, Dr. Sci. (Med.)

249036, Moscow Region, Obninsk, Koroleva Str., 4; 119048, Moscow, Trubetskaya Str., 8, building 2

dr.dga@mail.ruЯкименкоВ. А.YakimenkoV. A.

Якименко Владислав Андреевич

119048, Москва, ул. Трубецкая, 8, стр. 2

Vladislav A. Yakimenko

119048, Moscow, Trubetskaya Str., 8, building 2

Yavladislav87@gmail.comВадюхинМ. А.VadyukhinM. A.

Вадюхин Матвей Анатольевич

119048,  Москва, ул. Трубецкая, 8, стр. 2

Matvey A. Vadyukhin

119048, Moscow, Trubetskaya Str., 8, building 2

vma20@mail.ruУгурчиеваД. И.UgurchievaD. I.

Угурчиева Дали Ибрагимовна

119048, Москва, ул. Трубецкая, 8, стр. 2

Dali I. Ugurchieva

119048, Moscow, Trubetskaya Str., 8, building 2

daliyagurchieva@gmail.comКорякинС. Н.KoryakinS. N.

Корякин Сергей Николаевич, к.б.н.

249036, Московская обл., Обнинск, ул. Королева, 4 

Sergey N. Koryakin, Cand. Sci. (Biol.)

249036, Moscow Region, Obninsk, Koroleva Str., 4 

korsernic@mail.ruМедицинский радиологический научный центр им. А.Ф. Цыба — филиал ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава России; ФГАОУ ВО «Первый МГМУ им. И.М. Сеченова» Минздрава России (Сеченовский Университет)РоссияMedical Radiological Research Center named after A.F. Tsyba — Branch of the National Medical Research Center of Radiology; I.M. Sechenov First Moscow State Medical University of the Ministry of Health Care of Russia (Sechenov University)Russian FederationФГАОУ ВО «Первый МГМУ им. И.М. Сеченова» Минздрава России (Сеченовский Университет)РоссияI.M. Sechenov First Moscow State Medical University of the Ministry of Health Care of Russia (Sechenov University)Russian FederationМедицинский радиологический научный центр им. А.Ф. Цыба — филиал ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава РоссииРоссияMedical Radiological Research Center named after A.F. Tsyba — Branch of the National Medical Research Center of RadiologyRussian Federation2024101220242047077Copyright © Демяшкин Г.А., Якименко В.А., Вадюхин М.А., Угурчиева Д.И., Корякин С.Н., 20242024Демяшкин Г.А., Якименко В.А., Вадюхин М.А., Угурчиева Д.И., Корякин С.Н.Demyashkin G.А., Yakimenko V.A., Vadyukhin M.A., Ugurchieva D.I., Koryakin S.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1674

Облучение злокачественных новообразований печени и органов брюшной полости может приводить к развитию лучевого гепатита и печёночной недостаточности. Кроме того, острая воспалительная реакция стимулирует коллагеногенез, что вызывает радиационно-индуцированный фиброз. Интересным представляется исследование протекторных свойств аскорбиновой кислоты, которая является доказанным антиоксидантом и потенциально может снизить степень радиационно-индуцированного фиброза печени. Цель исследования – оценка степени радиационно-индуцированного фиброза печени в модели хронического лучевого гепатита и на фоне введения аскорбиновой кислоты. Крысы популяции линий Wistar (n=40) были поделены на четыре экспериментальные группы: I — контрольная (n=10); II — облучение электронами (n=10); III — введение аскорбиновой кислоты перед облучением электронами (n=10); IV — введение аскорбиновой кислоты (n=10). Животных всех групп (I–IV) выводили из эксперимента через 3 мес. после последней фракции. Проводили гистологическое исследование фрагментов печени и гистохимическое окрашивание по Массону. При гистохимическом окрашивании фрагментов печени по Массону на третьем месяце эксперимента обнаружили, что площадь коллагеновых и ретикулярных волокон, окрашенных анилиновым синим, практически сохраняла контрольные значения в группе введения аскорбиновой кислоты перед воздействием электронами, в то время как в группе облучения отмечали разрастание стромального компонента на фоне признаков лучевого гепатита. Введение аскорбиновой кислоты перед воздействием фракционного локального облучения электронами в суммарной дозе 30 Гр (модель лучевого гепатита) приводит к замедлению развития и снижению степени выраженности радиационно-индуцированного фиброза печени на третьем месяце эксперимента.

Irradiation of malignant neoplasms of the liver and abdominal organs can lead to the development of radiation hepatitis and liver failure. In addition, the acute inflammatory response stimulates collagenogenesis, which causes radiation-induced fibrosis. It appears promising to study the protective properties of ascorbic acid, which is a proven antioxidant capable of reducing the degree of radiation-induced liver fibrosis. In this study, we aim to assess the degree of radiation-induced liver fibrosis both in a model of chronic radiation hepatitis and against the background of administration of ascorbic acid. Wistar rats (n=40) were divided into four experimental groups: I – control (n=10); II – electron irradiation (n=10); III – administration of ascorbic acid before electron irradiation (n=10); IV – administration of ascorbic acid (n=10). Animals of all groups (I–IV) were removed from the experiment three months after the last fraction. A histological examination of liver fragments and histochemical staining according to Masson were performed. When performing histochemical staining of liver fragments according to Masson in the third month of the experiment, it was found that the area of collagen and reticular fibers stained with aniline blue practically retained control values in the group of pre-irradiation administration of ascorbic acid. For comparison, in the group of electron irradiation, a proliferation of the fibrous component was noted against the background of radiation hepatitis signs. Pre-irradiation administration of ascorbic acid slows down the development and decrease the severity of radiation-induced liver fibrosis three months after fractional local irradiation with electrons at a total dose of 30 Gy (in a model of radiation hepatitis).

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The authors declare that there are no conflicts of interest present.