scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-21-1-34-45scbmt-1698Research ArticleМЕТОДЫ И ТЕХНОЛОГИИ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙMETHODS AND TECHNOLOGIES OF BIOMEDICAL RESEARCHЭффекты высоких концентраций D-глюкозы на активность АМПА и НМДА рецепторов при моделировании гипергликемии на срезах мозгаEffects of Elevated D-Glucose Concentrations on AMPA and NMDA Re ceptor Activity in Modeled Hyperglycemia in Brain SlicesМокрушинА. А.MokrushinA. A.

Мокрушин Анатолий Александрович, д.б.н.

199034, Санкт-Петербург, наб. Макарова, 6

Anatoliy A. Mokrushin, Dr. Sci. (Biol.)

19903, St. Petersburg, Makarova Emb., 6

mok@inbox.ruФГБУН «Институт физиологии им. И.П. Павлова» РАНРоссияI.P. Pavlov Institute of Physiology of the Russian Academy of SciencesRussian Federation2025210420252113445Copyright © Мокрушин А.А., 20252025Мокрушин А.А.Mokrushin A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1698

На переживающих срезах мозга обонятельной коры мозга крыс исследовали влияния различных концентраций D-глюкозы (0,5; 1; 3; 5; 7; 10; 12; 14; 17; 20; 22; 25 мМ) на изменения активностей АМПА и НМДА ионотропных глутаматергических механизмов. Зависимость амплитуд АМПА и НМДА потенциалов от концентрации D-глюкозы была куполообразной. Малые концентрации (0,5; 1; 3; 5 мМ) вызывали прогрессивное увеличение амплитуд АМПА и НМДА потенциалов. При концентрации глюкозы во внеклеточной среде 7, 10 мМ амплитуды АМПА и НМДА потенциалов были максимальными и стабильными. При концентрации D-глюкозы 14 мМ активности АМПА и НМДА механизмов снижались и при дальнейшем увеличении углевода необратимо блокировались. Долго временная посттетаническая потенциация (модель неассоциативного обучения) развивалась только при концентрации D-глюкозы 10 мМ. Белок теплового шока (Мв 70 кДа) протектировал активности АМПА и НМДА механизмов от негативного действия высокой гипергликемической концентрации D-глюкозы 14 мМ. Полученные данные показывают реакции АМПА и НМДА механизмов при раз витии гипергликемии. Данную модель можно использовать для поиска веществ при защите нейрональных механизмов в нервной ткани при развитии гипергликемического сахарного диабета.

The effects of various D-glucose concentrations (0.5; 1; 3; 5; 7; 10; 12; 14; 17; 20; 22; and 25 mM) were studied using brain slices of the rat olfactory cortex to determine changes in the activities of AMPA and NMDA ionotropic glutamatergic mechanisms. The dependence of the amplitudes of the AMPA and NMDA potentials on D-glucose concentrations was dome-shaped. Lower concentrations (0.5; 1; 3; 5 mM) caused a progressive increase in the amplitudes of AMPA and NMDA potentials. Under D-glucose concentra tions in the extracellular medium of 7 and 10 mM, the amplitudes of AMPA and NMDA potentials were maximal and stable. Under a D-glucose concentration of 14 mM, the activities of AMPA and NMDA mechanisms decreased and, following a further increase in carbon, were irreversibly blocked. Long-term post-tetanic potentiation (model of non-associative learning) developed only at a D-glucose concentration of 10 mM. Heat shock protein (Mw70 kDa) protected the activities of AMPA and NMDA mechanisms from the negative effects of high hyperglycemic D-glucose concentration of 14 mM. The data obtained indicate the response of AMPA and NMDA mechanisms during the development of hyperglycemia. This model can be used to search for substances to protect neuronal mechanisms in the nervous tissue during the develop ment of hyperglycemic diabetes mellitus.

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The authors declare that there are no conflicts of interest present.