scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-21-4-96-100scbmt-1804Research ArticleРЕЛЕВАНТНОЕ И АЛЬТЕРНАТИВНОЕ БИОМОДЕЛИРОВАНИЕRELEVANT AND ALTERNATIVE BIOMODELLINGБиомоделирование воспалительно-амлоидного каскада как основы развития болезни Альцгеймера у старых мышей C57BL/6J.Biomodeling the Inflammatory Amyloid Cascade as the Basis of Alzheimer's Disease in Old C57BL/6J MicИнокентьеваВ. И.InokentevaV. I.

Инокентьева Виктория Игоревна

125315, Москва, ул. Балтийская, 8

Victoria I. Inokenteva

125315, Moscow, Baltiyskaya Str., 8

inokenteva_vi@academpharm.ruГруденьМ. А.GrudenM. A.

Грудень Марина Алексеевна, к.х.н.

125315, Москва, ул. Балтийская, 8

Marina A. Gruden, Cand. Sci. (Chem.)

125315, Moscow, Baltiyskaya Str., 8

gruden_ma@academpharm.ruСоловьеваО. А.SolovievaO. A.

Соловьева Ольга Александровна

125315, Москва, ул. Балтийская, 8

Olga A. Solovieva

125315, Moscow, Baltiyskaya Str., 8

solovieva_oa@academpharm.ruМихайловаН. П.MikhailovaN. P.

Михайлова Наталия Павловна

125315, Москва, ул. Балтийская, 8

Nataliya P. Mikhailova

125315, Moscow, Baltiyskaya Str., 8

mikhajlova_np@academpharm.ruРатмировА. М.RatmirovA. M.

Ратмиров Александр Максимович

125315, Москва, ул. Балтийская, 8

Alexander M. Ratmirov

125315, Moscow, Baltiyskaya Str., 8

ratmirov_am@academpharm.ruСторожеваЗ. И.StorozhevaZ. I.

Сторожева Зинаида Ивановна, д.б.н.

125315, Москва, ул. Балтийская, 8

Zinaida I. Storozheva, Dr. Sci. (Biol.)

125315, Moscow, Baltiyskaya Str., 8

storozheva_zi@academpharm.ruФГБНУ «Федеральный исследовательский центр оригинальных и перспективных биомедицинских и фармацевтических технологий»РоссияFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical TechnologiesRussian Federation20251812202521496100Copyright © Инокентьева В.И., Грудень М.А., Соловьева О.А., Михайлова Н.П., Ратмиров А.М., Сторожева З.И., 20252025Инокентьева В.И., Грудень М.А., Соловьева О.А., Михайлова Н.П., Ратмиров А.М., Сторожева З.И.Inokenteva V.I., Gruden M.A., Solovieva O.A., Mikhailova N.P., Ratmirov A.M., Storozheva Z.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1804

В данной работе было изучено влияние хронического интраназального введения олигомеров провоспалительного белка S100A9, индуцирующих воспалительно-амилоидный каскад, у 24-месячных мышей C57BL/6J на формирование и сохранность долговременной пространственной памяти, экспрессию гена Th, а также содержание и обмен дофамина в гиппокампе, фронтальной коре и мозжечке. Обнаружено нарушение долговременной памяти в 3-й и 4-й дни обучения и в сеансе тестирования в водном лабиринте Морриса у опытных животных по сравнению с контролем. Экспрессия Th статистически значимо повышалась в мозжечке, а содержание дофамина достоверно снижалось в гиппокампе. Таким образом, хроническое интраназальное введение олигомеров S100A9 старым животным способствует формированию патологических симптомов, подобных болезни Альцгеймера, и может рассматриваться в качестве разрабатываемой модели этого заболевания.

In this work, the effect of chronic intranasal administration of S100A9 proinflammatory protein oligomers, inducing the inflammatory-amyloid cascade, in 24-month-old C57BL/6J mice on the formation and preservation of long-term spatial memory, Th gene expression, as well as dopamine content and metabolism in the hippocampus, frontal cortex, and cerebellum was studied. Compared to the control, experimental animals showed an impairment of the long-term memory on days 3 and 4 of training and testing session in a Morris water maze. Th expression increased significantly in the cerebellum, while dopamine levels decreased significantly in the hippocampus. Thus, chronic intranasal administration of S100A9 oligomers to old animals contributes to the formation of pathological symptoms similar to Alzheimer's disease, thus showing potential as a model of this disease.

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The authors declare that there are no conflicts of interest present.