scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agency10.33647/2074-5982-22-1-70-83scbmt-1832Research ArticleБИОТЕХНОЛОГИИ В БИОМЕДИЦИНЕBIOTECHNOLOGIES IN BIOMEDICINEВлияние хитозана и энтеросорбентов на активность противомикробных препаратов in vitroEffect of Chitosan on the Activity of Antimicrobial Agents in vitroБеляеваА. Д.BeliaevaA. D.

Беляева Анна Дмитриевна

190013, Российская Федерация, Санкт-Петербург, Московский пр., 26

Anna D. Beliaeva

190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26

belyaeva_any@mail.ruНяниковаГ. Г.NianikovaG. G.

Няникова Галина Геннадьевна, к.б.н.. доц.,

190013, Российская Федерация, Санкт-Петербург, Московский пр., 26

Galina G. Nianikova, Cand. Sci. (Biol.), Assoc. Prof

190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26

galanyan@mail.ruСахиповаА. Р.SakhipovaA. R.

Сахипова Альфия Ринатовна

190013, Российская Федерация, Санкт-Петербург, Московский пр., 26

Alfiia R. Sakhipova

190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26 

alfiya.sakhipova@mail.ruБеляеваИ. Д.BeliaevaI. D.

Беляева Ирина Дмитривена

190013, Российская Федерация, Санкт-Петербург, Московский пр., 26 

 Irina D. Beliaeva 

190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26

belyaevairina97@gmail.comХарьковаО. Ю.KharkovaO. Iu.

Харькова Ольга Юрьевна

190013, Российская Федерация, Санкт-Петербург, Московский пр., 26

Irina D. Beliaeva

190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26

olga.kharkova.04@gmail.comФГБОУ ВО «Санкт-Петербургский государственный технологический институт (технический университет)»РоссияSaint Petersburg Institute of Technology (Technical University)Russian Federation2026140620262217083Copyright © Беляева А.Д., Няникова Г.Г., Сахипова А.Р., Беляева И.Д., Харькова О.Ю., 20262026Беляева А.Д., Няникова Г.Г., Сахипова А.Р., Беляева И.Д., Харькова О.Ю.Beliaeva A.D., Nianikova G.G., Sakhipova A.R., Beliaeva I.D., Kharkova O.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/1832

Проведено исследование влияния хитозана на эффективность антимикробных препаратов в условиях in vitro. Установлено, что энтеросорбенты «Лактофильтрум»®, «Хитозан Эвалар»® и «Энтеросгель»® не оказывают значимого влияния на активность антибиотиков, таких как ципрофлоксацин, амоксиклав, кларитромицин и цефоперазон. Низкомолекулярный крабовый хитозан (молекулярная масса (ММ): 50 кДа; степень деацетилирования (СД): 85,0%) повысил эффективность аминогликозидов (гентамицина и канамицина), макролидов (кларитромицина) и фтохинолонов (ломефлоксацина и офлоксацина). Среднемолекулярный крабовый хитозан (ММ: 83,7 кДа; СД: 89,0%) показал снижение активности офлоксацина и ломефлоксацина на 26,7 и 24,1% соответственно. Высокомолекулярный грибной хитозан из Rhizopus oryzae F-814 (ММ: 400 кДа; СД: 86,8%; ATCC 9363, NRRL 395, IMI 40564) продемонстрировал увеличение эффективности офлоксацина на 25,4–57,1%, что делает его перспективным для фармакологического применения. Предварительная сорбция антибиотиков на хитозане в течение 2 ч усиливала антимикробные эффекты, наблюдающиеся при последовательном внесении растворов в лунки.

The effect of chitosan on the efficacy of antimicrobial drugs was studied in vitro. It was established that the enterosorbents Lactofiltrum®, Chitosan Evalar®, and Enterosgel® exert no significant impact on the activity of antibiotics such as ciprofloxacin, amoxiclav, clarithromycin, and cefoperazone. Low-molecularweight crab chitosan (molecular weight (MW): 50 kDa; degree of deacetylation (DD): 85.0%) was found to enhance the efficacy of aminoglycosides (gentamicin and kanamycin), macrolides (clarithromycin), and fluoroquinolones (lomefloxacin and ofloxacin). Medium-molecular-weight crab chitosan (MW: 83.7 kDa; DD: 89.0%) showed a reduction in ofloxacin and lomefloxacin activity by 26.7% and 24.1%, respectively. High-molecular-weight fungal chitosan derived from Rhizopus oryzae F-814 (MW: 400 kDa; DD: 86.8%; ATCC 9363, NRRL 395, IMI 40564) exhibited a 25.4%–57.1% increase in ofloxacin efficacy, highlighting its potential for pharmacological applications. Preliminary sorption of antibiotics onto chitosan for two hours enhanced the antimicrobial effects observed under sequential administration of antibiotic solutions into assay

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The authors declare that there are no conflicts of interest present.