scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agencyscbmt-508Research ArticleМЕТОДЫ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙMETHODS OF BIOMEDICAL RESEARCHESЭффекты разных концентраций бактериального меланина на электрическую активность нейронов коры мозга при раздражении нервов задней конечности у крысEffects of different concentrations of bacterial melanin on the electrical activity of neurons in the cerebral cortex in response to stimulation of the rat hindlimb nervesПетросянТ. Р.PetrosyanT. R.tigpetrosyan@mail.ruЧавушянВ. А.ChavushyanV. A.noemail@neicon.ruТер-МаркосянА. С.Ter-MarkosyanA. S.noemail@neicon.ruОвсепянА. С.HovsepianA. S.noemail@neicon.ruЕреванский государственный медицинский университетRussian FederationИнститут физиологии НАН РАRussian FederationНПЦ «Армбиотехнология» ГНКО НАН РАRussian Federation201522012020135261Copyright © Петросян Т.Р., Чавушян В.А., Тер-Маркосян А.С., Овсепян А.С., 20202020Петросян Т.Р., Чавушян В.А., Тер-Маркосян А.С., Овсепян А.С.Petrosyan T.R., Chavushyan V.A., Ter-Markosyan A.S., Hovsepian A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/508

С целью выявления сдвигов в электрической постстимульной активности нейронов сенсомоторной коры мозга (СМК) под влиянием разных концентраций бактериального меланина изучалась высокочастотная тетаническая активация корковых нейронов в ответ на стимуляцию периферических нервов задней конечности у крыс. Регистрировались соответствующие посттетанические короткие (SТР) и/или длительные (LТР) изменения активности корковых нейронов, связанных с мобилизацией того или иного медиатора изучаемой структуры. Характер воздействия раствора меланина оказался прямо зависящим от исследуемой концентрации раствора. Оптимальным для данной работы признан способ внутримышечного введения. Картины посттетанических ответов практически идентичны при прямой аппликации и внутримышечном и/или внутрибрюшинном введении меланина при применении одной и той же концентрации. При введении низких концентраций раствора меланина отмечалось повышение нейрональной активности. Самой надежной концентрацией является 4,5 мг/мл, которая вызывает стабильное и длительное повышение активности корковых нейронов. Активирующее влияние бактериального меланина может способствовать процессам восстановления при нейродегенеративных заболеваниях.

In order to detect changes in the post-stimulus electrical activity of neurons in sensorimotor cortex (SMC) induced by the influence of different concentrations of bacterial melanin, high-frequency tetanic activation of cortical neurons was studied in response to stimulation of peripheral nerves of rat hindlimb. The corresponding post-tetanic short (STP) and/or long-term (LTP) changes in the activity of cortical neurons, caused by the mobilization of a mediator, were registered. The nature of the impact was directly dependent on the concentration of the tested solution. The method of intramuscular injection is recognized as the best method for this research. The pattern of posttetanic responses was almost identical after direct application and intramuscular and/or intraperitoneal injection of melanin at use of the same concentration. The increase of neuronal activity was noted by introduction of low concentration of solution of melanin. The most reliable concentration is the concentration of 4.5 mg/ml, which induces stable and long-lasting increase in the activity of the cortical neurons. Activating effect of bacterial melanin can contribute to the recovery processes in neurodegenerative diseases.

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The authors declare that there are no conflicts of interest present.