scbmtБИОМЕДИЦИНАJournal Biomed2074-59822713-0428Scientific center of biomedical technologies of Federal Medical and Biological Agencyscbmt-514Research ArticleНОВЫЕ БИОМЕДИЦИНСКИЕ ТЕХНОЛОГИИNEW BIOMEDICAL TECHNOLOGIESЭкспрессия гипоксия-индуцибельного фактора HIF1α как критерий развития гипоксии тканейThe expression of hypoxia-inducible factor HIF1α as a criterion for the development of tissue hypoxiaШустовЕ. Б.ShustovE. B.shustov-msk@mail.ruКаркищенкоН. Н.KarkischenkoN. N.noemail@neicon.ruДуляМ. С.DulyaM. S.noemail@neicon.ruСеменовХ. Х.SemenovKh. Kh.noemail@neicon.ruОковитыйС. В.OkovitiyS. V.noemail@neicon.ruРадькоС. В.RadkoS. V.noemail@neicon.ruФГБУН «Научный центр биомедицинских технологий ФМБА России»Russian FederationФГБОУ ВПО «Санкт-Петербургская химико-фармацевтическая академия МЗ РФ»Russian Federation20152201202014415Copyright © Шустов Е.Б., Каркищенко Н.Н., Дуля М.С., Семенов Х.Х., Оковитый С.В., Радько С.В., 20202020Шустов Е.Б., Каркищенко Н.Н., Дуля М.С., Семенов Х.Х., Оковитый С.В., Радько С.В.Shustov E.B., Karkischenko N.N., Dulya M.S., Semenov K.K., Okovitiy S.V., Radko S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.scbmt.ru/jour/article/view/514

Целью исследования была оценка пригодности применения уровня экспрессии гипоксия-индуцированного фактора HIF1α для диагностики степени тяжести гипоксии тканей. Экспрессия HIF1α является типовой ответной реакцией генома клеток на гипоксическое воздействие, запускающей многоплановые механизмы адаптации к гипоксии и повышения способности тканей к переживанию неблагоприятных воздействий. Экспериментальная гипоксия создавалась подъемом лабораторных крыс Wistar в барокамере на высоты 6, 8, 10, 11 и 12 км. Длительность экспозиции варьировала от 30 до 5 минут в зависимости от высоты. Сразу же после спуска животных забирались биологические материалы (цельная кровь, почки, печень, сердце, мозг), в которых методом ПЦР в реальном времени определялась экспрессия HIF1α. В качестве гена стандартизации использовался ген «домашнего хозяйства» TSPO (митохондриальный транслокаторный белок). Показано, что и HIF1α, и TSPO обладают выраженной тканеспецифичностью. В условиях гипоксии экспрессия TSPO по высотам характеризуется высокой вариативностью. Динамика экспрессии HIF1α по высотам показывает принципиальную возможность использования этого показателя для оценки выраженности тканевой гипоксии, не зависящей от способа моделирования гипоксического воздействия.

The aim of the study was to evaluate the suitability of the use of the expression level of hypoxia-inducible factor HIF1α to diagnose the severity of tissue hypoxia. Expression HIF1α is a typical response of the genome of the cells in the hypoxic effects, triggering multidimensional mechanisms of adaptation to hypoxia, and improve the ability of tissues to experience adverse effects. Experimental hypoxia was created by the rise of laboratory Wistar rats in the chamber at a height of 6, 8, 10, 11 and 12 km. Duration of exposure varied from 30 to 5 minutes depending on altitude. Immediately after the descent of animals climbed the biological materials (whole blood, kidney, liver, heart, brain), in which method the real-time PCR determined the expression of HIF1a. As the standardization gene used was the gene "household" TSPO. It is shown that HIF1a, and TSPO have pronounced tissue-specific. Under hypoxic conditions the expression of TSPO elevation is characterized by high variability. Dynamics of expression of HIF1a elevation shows the possibility of using this indicator to assess the severity of tissue hypoxia-independent method of modeling hypoxic exposure.

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The authors declare that there are no conflicts of interest present.