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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">scbmt</journal-id><journal-title-group><journal-title xml:lang="ru">БИОМЕДИЦИНА</journal-title><trans-title-group xml:lang="en"><trans-title>Journal Biomed</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2074-5982</issn><issn pub-type="epub">2713-0428</issn><publisher><publisher-name>Scientific center of biomedical technologies of Federal Medical and Biological Agency</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">scbmt-97</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МЕТОДЫ БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>METHODS OF BIOMEDICAL RESEARCHES</subject></subj-group></article-categories><title-group><article-title>Влияние холодной гелиевой плазмы на метаболические и физико-химические параметры крови человека invitro</article-title><trans-title-group xml:lang="en"><trans-title>The influence of helium cold plasma on metabolic and physical-chemical parameters of human blood in vitro</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мартусевич</surname><given-names>А. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Martusevich</surname><given-names>A. K.</given-names></name></name-alternatives><email xlink:type="simple">cryst-mart@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Соловьева</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Soloveva</surname><given-names>A. G.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Краснова</surname><given-names>С. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Krasnova</surname><given-names>S. Yu.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Янин</surname><given-names>Д. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Yanin</surname><given-names>D. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Галка</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Galka</surname><given-names>A. G.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Костров</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kostrov</surname><given-names>A. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>ФГБУ «Приволжский федеральный медицинский исследовательский центр» Минздрава России, Нижний Новгород; Ассоциация российских озонотерапевтов</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-2"><institution>ФГБУ «Приволжский федеральный медицинский исследовательский центр» Минздрава России</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-3"><institution>ФИЦ «Институт прикладной физики РАН»</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>26</day><month>02</month><year>2019</year></pub-date><volume>0</volume><issue>2</issue><fpage>47</fpage><lpage>58</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Мартусевич А.К., Соловьева А.Г., Краснова С.Ю., Янин Д.В., Галка А.Г., Костров А.В., 2019</copyright-statement><copyright-year>2019</copyright-year><copyright-holder xml:lang="ru">Мартусевич А.К., Соловьева А.Г., Краснова С.Ю., Янин Д.В., Галка А.Г., Костров А.В.</copyright-holder><copyright-holder xml:lang="en">Martusevich A.K., Soloveva A.G., Krasnova S.Y., Yanin D.V., Galka A.G., Kostrov A.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.scbmt.ru/jour/article/view/97">https://journal.scbmt.ru/jour/article/view/97</self-uri><abstract><p>Цель данного исследования - сравнительная оценка сдвигов окислительного метаболизма и кристаллогенных свойств плазмы крови при обработке гелиевой холодной плазмой и неионизированным потоком гелия. Изучали действие СВЧ-генерированной гелиевой холодной плазмы на образцы цельной крови человека. Продолжительность воздействия составляла 1 и 3 мин. Для проведения эксперимента образцы крови делили на 5 равных порций по 1,5 мл, причем первая из них являлась контрольной (с ней не осуществляли никаких манипуляций), вторую и третью обрабатывали холодной плазмой с указанными выше экспозициями, а четвертую и пятую - потоком гелия без перевода его в плазменную форму. В плазме крови всех образцов исследовали параметры окислительного метаболизма методом Fe-индуцированной биохемилюминесценции и кристаллогенную активность. Установлено, что холодная гелиевая плазма и неионизированный поток гелия оказывают модифицирующее влияние на окислительный метаболизм и кристаллогенные свойства плазмы крови при обработке in vitro . Для холодной плазмы оно проявилось в антиоксидантном эффекте и стимуляции кристаллогенной активности, тогда как у потока гелия обнаружено прооксидантное действие и способность угнетать дегидратационную структуризацию биосреды. При этом наиболее оптимальной для действия холодной плазмы является 1-минутная экспозиция.</p></abstract><trans-abstract xml:lang="en"><p>The aim of the study was comparative estimation of the changes of oxidative metabolism and crystallogenic properties of blood plasma under processing with cold helium plasma and non-ionized helium flow. We studied the influence of microwave-generating cold plasma on the specimens of whole human blood. The exposure time was 1 and 3 min. Before processing all blood specimens were divided into 5 portions. First portion was control (without any manipulations), second and third portion were treated with cold plasma, fourth and fifth ones were sparged with non-ionized helium flow. In all portions we estimated the parameters of oxidative metabolism and crystallogenic activity. It was stated that cold helium plasma and non-ionized helium modified these parameters under blood processing in vitro. For cold helium plasma this effect was realized by stimulation of antioxidant activity and crystallogenic properties of blood plasma. In opposite, non-ionized helium flow had prooxidant effect and demonstrated the inhibition of biological fluid crystallization. Our data showed that most optimal time for blood processing with cold plasma is 1 min.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>холодная плазма</kwd><kwd>биологические эффекты</kwd><kwd>плазма крови</kwd><kwd>окислительный метаболизм</kwd><kwd>кристаллогеные свойства</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cold plasma</kwd><kwd>biological effects</kwd><kwd>blood plasma</kwd><kwd>oxidative metabolism</kwd><kwd>crystallogenic properties</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Алейник А.Н. Плазменная медицина. - Томск: Изд-во ТПУ, 2011. 45 с.</mixed-citation><mixed-citation xml:lang="en">Алейник А.Н. Плазменная медицина. - Томск: Изд-во ТПУ, 2011. 45 с.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Балданов Б.Б., Ранжуров Ц.Р., Норбоев Ч.Н. и др. 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