Optimizing the Hormonal Mice Treatment Protocol to Induce Superovulation
https://doi.org/10.33647/2074-5982-16-3-48-53
Abstract
Mice treatment with pregnant mare gonadotropin serum (PMGS) in combination with human chorionic gonadotropin (hCG) is considered to be the “golden standard” of the induced superovulation procedure. The effect of administering the same doses may differ depending on the manufacturer of the preparation. According to our results, the use of PMGS produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female. At the same time, the working dose of Follimagum (Mosagrogen, Russia) was not determined. The result comparable with that of Synchrostim 500 and Sergon was achieved using a composite drug Menopur (Ferring, Germany). The study of the influence of mice age on the hormonal response showed 3–4 weeks to be the most productive age, when the maximal number of viable embryos was obtained under a minimal external invasion.
About the Authors
E. S. Savchenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Elena S. Savchenko
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
N. S. Ogneva
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nastasya S. Ogneva
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
S. V. Maksimenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Sergey V. Maksimenko, Cand. Sci. (Biol.)
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
M. M. Skripkina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Maria M. Skripkina
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
N. V. Petrova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Natalya V. Petrova
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
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Review
For citations:
Savchenko E.S., Ogneva N.S., Maksimenko S.V., Skripkina M.M., Petrova N.V. Optimizing the Hormonal Mice Treatment Protocol to Induce Superovulation. Journal Biomed. 2020;16(3):48-53. (In Russ.) https://doi.org/10.33647/2074-5982-16-3-48-53
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