Methodical aspects of lipopolysaccharides obtaining and characterization from Escherichia coli cells
https://doi.org/10.33647/2713-0428-17-3E-14-16
Abstract
In this study, a procedure was applied to purify lipopolysaccharides from Escherichia coli based on a hot phenolic extraction protocol. The purity of the extracted lipopolysaccharides was assessed by HPLC-UV. Pyrogenic activity was determined using the Limulus Amebocyte Lysate test and used to monitor the functionality of the purified lipopolysaccharides. HPLC analysis showed a high degree of purity comparable to commercial lipopolysaccharide. Pyrogenic activity confirmed the functional activity of purified lipopolysaccharides. The presented protocol can be used to isolate lipopolysaccharides with high purity and functional activity.
About the Authors
R. A. Ageldinov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
A. I. Levashova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Cand. Sci. (Chem.),
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
V. S. Kokhan
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
M. S. Nesterov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
References
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Review
For citations:
Ageldinov R.A., Levashova A.I., Kokhan V.S., Nesterov M.S. Methodical aspects of lipopolysaccharides obtaining and characterization from Escherichia coli cells. Journal Biomed. 2021;17(3E):14-16. (In Russ.) https://doi.org/10.33647/2713-0428-17-3E-14-16
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