Genotyping of mice lines for the TPH2 gene in routine practice: selection of a DNA extraction technique for PCR
https://doi.org/10.33647/2713-0428-17-3E-48-52
Abstract
The development of a fast and highly accurate genotyping protocol is crucial for the use of biomodels with TPH2 gene knockout in experiments. Genotyping of mutant mice is currently carried out using methods that include sequencing or multi-stage PCR. These methods are expensive and time-consuming. This paper presents a comparative analysis of two methods: detection of amplification products by gel electrophoregram and real-time PCR. The procedure for extracting DNA from fragments of mice tails is also considered.
About the Authors
K. A. Kurbakov
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences
Russian Federation
Russian Federation
109316, Moscow, Talalikhina Str., 26
A. A. Kibitkina
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences
Russian Federation
Russian Federation
109316, Moscow, Talalikhina Str., 26
L. V. Fedilova
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences
Russian Federation
Russian Federation
Cand. Sci. (Tech.),
109316, Moscow, Talalikhina Str., 26
E. R. Vasilevskaya
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences
Russian Federation
Russian Federation
Cand. Sci. (Tech.),
109316, Moscow, Talalikhina Str., 26
G. S. Tolmacheva
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences
Russian Federation
Russian Federation
109316, Moscow, Talalikhina Str., 26
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Review
For citations:
Kurbakov K.A., Kibitkina A.A., Fedilova L.V., Vasilevskaya E.R., Tolmacheva G.S. Genotyping of mice lines for the TPH2 gene in routine practice: selection of a DNA extraction technique for PCR. Journal Biomed. 2021;17(3E):48-52. (In Russ.) https://doi.org/10.33647/2713-0428-17-3E-48-52
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