Comparison of exosome isolation methods for biomedical research
https://doi.org/10.33647/2713-0428-17-3E-76-79
Abstract
Exosomes are nanoscale membrane vesicles secreted by almost all kinds of cells and stably existing in the body. Due to their unique properties, exosomes can be used in biomedical research as regenerative preparations or drug delivery vehicles. In this study, an anion exchange (AIEX) chromatography-based protocol for isolation of extracellular vesicles from a cell-conditioned medium was developed. The exosome isolation method based on AIEX chromatography overperforms the canonical ultracentrifugation-based isolation method in terms of its capability of large-volume processing.
About the Authors
N. I. Ponomareva
National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health Сare of Russia;
I.M. Sechenov Moscow State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
127473, Moscow, Dostoevskaya Str., 4, Building 2;
119991, Moscow, Trubetskaya Str., 8, Building 2
A. P. Kostyusheva
National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
127473, Moscow, Dostoevskaya Str., 4, Building 2
S. A. Brezgin
National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
127473, Moscow, Dostoevskaya Str., 4, Building 2
V. V. Smirnov
I.M. Sechenov Moscow State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
119991, Moscow, Trubetskaya Str., 8, Building 2
V. I. Gegechkory
I.M. Sechenov Moscow State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
Cand. Sci. (Pharmaceutic.),
119991, Moscow, Trubetskaya Str., 8, Building 2
D. S. Kostyushev
National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health Сare of Russia;
Sirius University of Science and Technology
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
127473, Moscow, Dostoevskaya Str., 4, Building 2;
354340, Sochi, Olimpiyskiy Avenue, 1
V. P. Chulanov
National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health Сare of Russia;
I.M. Sechenov Moscow State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
Dr. Sci. (Med.),
127473, Moscow, Dostoevskaya Str., 4, Building 2;
119991, Moscow, Trubetskaya Str., 8, Building 2
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Review
For citations:
Ponomareva N.I., Kostyusheva A.P., Brezgin S.A., Smirnov V.V., Gegechkory V.I., Kostyushev D.S., Chulanov V.P. Comparison of exosome isolation methods for biomedical research. Journal Biomed. 2021;17(3E):76-79. (In Russ.) https://doi.org/10.33647/2713-0428-17-3E-76-79
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