Preparation, Characterization and Preliminary in vivo Testing of Liposomated Antiidiotypic Antibodies Against Morphine Derivatives
https://doi.org/10.33647/2074-5982-17-4-8-17
Abstract
Artificial membrane structures containing medicinal substances are highly promising for the development of new drugs. Liposomal preparations are actively used in medical practice due to their high efficacy and relatively low toxicity. Our aim was to encapsulate anti-idiotypic antibodies into a liposomal composition with the purpose of improving their immunogenic properties. Following the preparation of a liposomal composition by the dehydration/rehydration method using ultrasonic treatment, the size, zeta potential, and loading efficiency of liposomes were investigated. Preliminary in vivo studies were conducted to evaluate the adjuvant properties of liposomes of varying size. Loaded liposomes of the smallest diameter (about 110 nm) showed the potential of enhancing the immune response similar to that obtained using Freund’s adjuvant. These results justify further research into the properties of liposomes loaded with antibodies.
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladislav N. Karkischenko, Dr. Sci. (Med.), Prof.
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
A. G. Berzina
V. Serbsky National Medical Research Centre for Psychiatry and Narcology of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Asya G. Berzina, Cand. Sci. (Biol.)
119034, Moscow, Kropotkinskiy Lane, 23
T. A. Klimova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Tatiana A. Klimova, Cand. Sci. (Biol.)
119034, Moscow, Kropotkinskiy Lane, 23
N. B. Gamaleya
V. Serbsky National Medical Research Centre for Psychiatry and Narcology of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Natalia B. Gamaleya, Dr. Sci. (Med.), Prof.
119034, Moscow, Kropotkinskiy Lane, 23
R. A. Ageldinov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia; Mendeleev University of Chemical Technology of Russia
Russian Federation
Russian Federation
Ruslan A. Ageldinov
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
125047, Moscow, Miusskaya Square, 9
A. E. Kuznetsov
Mendeleev University of Chemical Technology of Russia
Russian Federation
Russian Federation
Alexander E. Kuznetsov, Cand. Sci. (Tech.)
125047, Moscow, Miusskaya Square, 9
I. S. Korsun
Mendeleev University of Chemical Technology of Russia
Russian Federation
Russian Federation
Irina S. Korsun
125047, Moscow, Miusskaya Square, 9
M. S. Nesterov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Maksim S. Nesterov
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
L. I. Ulyanova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Lyudmila I. Ulyanova, Dr. Sci. (Biol.)
119034, Moscow, Kropotkinskiy Lane, 23
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Review
For citations:
Karkischenko V.N., Berzina A.G., Klimova T.A., Gamaleya N.B., Ageldinov R.A., Kuznetsov A.E., Korsun I.S., Nesterov M.S., Ulyanova L.I. Preparation, Characterization and Preliminary in vivo Testing of Liposomated Antiidiotypic Antibodies Against Morphine Derivatives. Journal Biomed. 2021;17(4):8-17. (In Russ.) https://doi.org/10.33647/2074-5982-17-4-8-17
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