Burn Wound Healing Activity of Prunella Grandiflora L. Exctract
https://doi.org/10.33647/2074-5982-17-4-50-56
Abstract
The qualitative composition of phenolic acids and burn wound healing activity of Prunella grandiflora L. extract were studied. The content of rosmarinic (621±5 mg/g), coffeic (11.2±0.2 mg/g), syringic (46.5±1.4 mg/g), n-coumaric (25.8±0.5 mg/g), and ferulic (42.7±2.9 mg/g) acids in dry extracts of P. grandiflora was determined by HPLC. Three new burn-healing agents of different composition containing 5% of the dry extract of Prunella grandiflora L. (big-flowered self-heal) were developed. The developed agents were tested on male Wistar rats with second- and third-degree thermal burns. An assessment of skin restoration was carried out in experimental and control groups of animals. It was revealed that animals treated with the developed soft dosage forms restored the epithelium and coat 6–8 days faster that those treated with compositions containing no extract under study. It was found that the developed soft dosage forms based on P. grandiflora extract exhibit anti-inflammatory and wound-healing action.
About the Authors
E. V. Bolotnik
Botanical Garden of the Ural Branch of RAS
Russian Federation
Russian Federation
Elizaveta V. Bolotnik, Cand. Sci. (Biol.)
620144 Ekaterinburg, 8 Marta Str., 202-а
L. I. Alekseeva
Botanical Garden of the Ural Branch of RAS
Russian Federation
Russian Federation
Lyudmila I. Alekseeva, Cand. Sci. (Chem.)
620144 Ekaterinburg, 8 Marta Str., 202-а
L. P. Larionov
Ural State Medical University
Russian Federation
Russian Federation
Leonid P. Larionov, Dr. Sci. (Med.), Prof.
620028, Ekaterinburg, Repina Str., 3
References
1. Azhikova A.K., Lazko M.V., Azhikov A.S. Terapevticheskij e’’ffekt ispol’’zovaniya kedrovogo masla pri ozhogovoj travme [The therapeutic effect of using cedar nut oil for burn injury]. Estestvenny’e nauki [Natural Sciences]. 2008;4(25):75–77. (In Russian).
2. Rosmarinic acid and antioxidant activity of Prunella grandiflora and Prunella vulgaris (Lamiaceae)]. Rastitel’ny’j mir Aziatskoj Rossii [The flora of Asian Russia]. 2013;11(1):121–125. (In Russian).
3. Budantsev A.A., Lesiovskaya E.E. Rozmarinovaya kislota: istochniki i biologicheskaya aktivnost [Rosemarinic acid: sources and biological activity]. Rastitel’ny’e resursy’ [Растительные ресурсы]. 2012;48(3):453–468. (In Russian).
4. Volkov A.A., Larionov L.P., Zabokriczkij N.A., Xonina T.G., Tosova I.N., Fedotova E.V., Mishechkina A.A., Kochetkova V.E., Tarasova E.V. Razrabotka i doklinicheskoe izuchenie innovacionny’x kompozicij myagkoj lekarstvennoj formy’ na osnove dimetildigliceroksisilana pri termicheskoj travme [Development and preclinical study of innovative compositions of soft dosage form based on dimethyldiglyceroxysilane for thermal injury]. Biomeditsina [Journal Biomed]. 2010;5:71–73. (In Russian).
5. Gavrilov A.S. Farmacevticheskaya texnologiya. Izgotovlenie lekarstvenny’x preparatov [Pharmaceutical technology. Manufacturing of medicines]. Мoscow: GE’OTARMedia Publ., 2010:624. (In Russian).
6. Guskova T.A. Toksikologiya lekarstvenny’h sredstv [Drug toxicology]. Moscow: Russkij vrach Publ., 2003:76. (In Russian).
7. Kurchatova M.N., Durnova N.A. Vliyanie rastitel’ny’h e’kstraktov na indukciyu mikroyader ciklofosfanom v e’ritrocitax krovi besporodny’x bely’x my’shej [Influence of plant extracts on the induction of micronuclei by cyclophosphamide in erythrocytes of blood of outbred white mice]. Cytology. 2015;57(6):452–458. (In Russian).
8. Lesiovskaya E.E. Dokazatel’naya fitoterapiya [Evidence-based herbal medicine]. Мoscow: Remedium Publ., 2014:224. (In Russian).
9. Khabriev R.U. Rukovodstvo po e’ksperimental’nomu (doklinicheskomu) izucheniyu novy’h farmakologicheskih veshhestv [Guidelines for experimental (preclinical) study of new pharmacological substances]. Moscow: Meditsina Publ., 2013:942. (In Russian).
10. Chmyrev I.V., Butrin Ya.L. Konservativnoe lechenie ozhogov licza [Conservative treatment of facial burns]. Medicinskij vestnik Yuga Rossii [Medical Bulletin of the South of Russia]. 2020;11(2):129–133. (In Russian).
11. Shamilov A.A. Rasteniya roda Prunella: himicheskij sostav, vidy’ farmakologicheskogo dejstviya [Plants of the Prunella genus: chemical composition, types of pharmacological action]. Voronezh State University Bulletin, Series: Chemistry, Biology, Pharmacy. 2017;4:152–160. (In Russian).
12. Qiang Z.,Ye Z., Hauck C., Murphy P.A., McCoy J.-A., Widrllechner M.P., Reddy M., Hendrich S. Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extract across Caco — 2 cell monolayers. J. of Etno pharmacology. 2011;137:1107–1112.
13. Oh C.S., Price J., Brindley M.A., Widrlechner M.P., Qu L., McCoy J.-A., Murphy P., Hauck C., Maury W. Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L. Virology J. 2011;8:188–197.
Review
For citations:
Bolotnik E.V., Alekseeva L.I., Larionov L.P. Burn Wound Healing Activity of Prunella Grandiflora L. Exctract. Journal Biomed. 2021;17(4):50-56. (In Russ.) https://doi.org/10.33647/2074-5982-17-4-50-56
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