Impact of Intranasal Insulin Administration On Na+/K+-Atpase and Са2+-Transporting System Components in Rat Cardiomyocytes with Type 1 Diabetes Mellitus
https://doi.org/10.33647/2074-5982-18-2-52-62
Abstract
Cardiovascular pathology is the main cause of morbidity among patients with diabetes mellitus. The development of a specific therapy aimed at either blunting the protein signals involved in pathological cardiomyocyte hypertrophy or upregulating the expression of cardioprotective pathways can support new strategies for treating diabetes-induced cardiac dysfunctions. The aim of the work was to study the impact of intranasal insulin administration (IIA) on the expression of genes encoding insulin-dependent signaling proteins and components of the Ca2+-transporting system, as well as on the activity of Na+/K+-ATPase in cardiomyocytes on the model of experimental type 1 diabetes mellitus (DM1) in rats. It was shown that IIA eliminates the uncoupling of molecular mechanisms involved in electromechanical coupling in rat cardiomyocytes that occurs under the conditions of mild DM1. This allowed us to recommend IIA as a therapeutic approach to the prevention and treatment of structural and functional myocardial disorders caused by diabetes.
Keywords
heart,
diabetes mellitus,
diabetic cardiomyopathy,
Na+/K+-ATPase,
intranasally administered insulin,
rat
Supporting agencies: Funding: State budget funds of the Ministry of Science and Higher Education assignment No. 075- 00886-20-01 (direction AAAA-A18-118012290427) using the equipment of the Center for Collective Use of the IEPhB RAS. Acknowledgment: The authors express their appreciation to Olga Sergeevna Tarasova, Prof. of Moscow State University, for discussing the ideas presented in the article.
About the Authors
I. B. Sukhov
Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
194223, Russian Federation, Saint-Petersburg, Thorez Ave., 44
O. V. Chistyakova
Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
194223, Russian Federation, Saint-Petersburg, Thorez Ave., 44
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For citations:
Sukhov I.B., Chistyakova O.V. Impact of Intranasal Insulin Administration On Na+/K+-Atpase and Са2+-Transporting System Components in Rat Cardiomyocytes with Type 1 Diabetes Mellitus. Journal Biomed. 2022;18(2):52-62. (In Russ.) https://doi.org/10.33647/2074-5982-18-2-52-62
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