Changes in Tissue Oxidative Metabolism Predict the Severity of Disorders in the System of Blood and Bone Marrow Cells in a Mouse Model of Type 2 Diabetes Mellitus
https://doi.org/10.33647/2713-0428-20-3E-107-112
Abstract
The progressive development of diabetes mellitus (DM2) in db/db mice is associated with three stages of impaired carbohydrate metabolism and redox processes. In db/db mice, the content of erythrocytes, hemoglobin, and leukocytes was found to decrease already in stage I of DM2. In the II and, in particular, III stages, an increase in platelets, percentage of neutrophils, monocytes, and eosinophils was observed, along with a decrease in lymphocytes. In the bone marrow of db/db mice, a decrease in the proportion of living cells and an increase in the number of damaged cells were determined already in stage I, but especially in stage III, mainly due to aponecrotic cells. Therefore, along with the progression of DM2 and a marked decrease in the effectiveness of redox processes, particularly in stage III, hematopoiesis processes are inhibited and disturbances in the ratio of neutrophil/lymphocyte cell populations increase. This indicates the development of severe hypoxia, activation of a systemic inflammatory reaction, and inhibition of reparative processes that create conditions for the development of dangerous complications.
Supporting agencies: the work was partially completed within the framework of the state assignment on the topic: “Assessment of the body's adaptive responses to the action of physicochemical and environmental factors” (No. FGFU-2022-0010).
About the Authors
O. I. Stepanova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Olga I. Stepanova, Cand. Sci. (Biol.)
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
R. A. Klesov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Roman A. Klesov
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
Kh. Kh. Semenov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Khyzyr Kh. Semenov, Cand. Sci. (Biol.)
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
I. B. Alchinova
Research Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Irina B. Alchinova, Cand. Sci. (Biol.)
125315, Moscow, Baltiyskaya Str., 8
A. B. Cherepov
Research Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Anton B. Cherepov
125315, Moscow, Baltiyskaya Str., 8
A. A. Metelkin
Research Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Arkady A. Metelkin
125315, Moscow, Baltiyskaya Str., 8
M. Yu. Karganov
Research Institute of General Pathology and Pathophysiology
Russian Federation
Russian Federation
Mikhail Yu. Karganov, Dr. Sci. (Biol.), Prof.
125315, Moscow, Baltiyskaya Str., 8
A. O. Nikolskaya
Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Alla O. Nikolskaya, Cand. Sci. (Biol.)
123182, Moscow, Shchukinskaya Str., 1
N. A. Onishchenko
Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Nina A. Onishchenko, Dr. Sci. (Med.), Prof.
123182, Moscow, Shchukinskaya Str., 1
Yu. B. Basok
Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Health Care of Russia
Russian Federation
Russian Federation
Yulia B. Basok, Dr. Sci. (Med.)
123182, Moscow, Shchukinskaya Str., 1
References
1. Stepanova O.I., Klesov R.A., Semenov Kh.Kh., Pomytkin I.A., Onishchenko N.A., Karkischenko V.N. Sposob neinvazivnogo izucheniya tkanevykh narusheniy pri sakharnom diabete 2 tipa u myshey db/db c pomoshch'yu lazernoy dopplerovskoy fluometrii [A method for noninvasive studying tissue disorders in type 2 diabetes mellitus in db/db mice using laser Doppler flowmetry]. Patologicheskaya fiziologiya i eksperimental'naya terapiya [Pathological Physiology and Experimental Therapy]. 2023;67(2):118–129. (In Russian). DOI: 10.25557/0031-2991.2023.02.118-129
2. Amend S.R., Valkenburg K.C., Pienta K.J. Murine hind limb long bone dissection and bone marrow isolation. J. Vis. Exp. 2016;110:53936. DOI: 10.3791/53936
3. Crowley L.C., Marfell B.J., Scott A.P., Waterhouse N.J. Quantitation of apoptosis and necrosis by annexin V binding, propidium iodide uptake, and flow cytometry. Cold Spring Harb Protoc. 2016;2016(11). DOI: 10.1101/pdb.prot087288
Review
For citations:
Stepanova O.I., Klesov R.A., Semenov Kh.Kh., Alchinova I.B., Cherepov A.B., Metelkin A.A., Karganov M.Yu., Nikolskaya A.O., Onishchenko N.A., Basok Yu.B. Changes in Tissue Oxidative Metabolism Predict the Severity of Disorders in the System of Blood and Bone Marrow Cells in a Mouse Model of Type 2 Diabetes Mellitus. Journal Biomed. 2024;20(3E):107-112. (In Russ.) https://doi.org/10.33647/2713-0428-20-3E-107-112
Views:
162
Email this article 
