Interaction of β-amyloid Peptide with Insulin Detemir Studied by Biolayer Interferometry and Molecular Modelling
https://doi.org/10.33647/2713-0428-20-3E-233-237
Abstract
The study of β-amyloid peptide (Aβ) interactions with protein and peptide ligands is a relevant research task. Aβ complexes with drugs that are considered as promising candidates for the therapy of Alzheimer’s disease are of particular interest. This category includes a group of hypoglycemic drugs, such as insulin detemir, a long-acting insulin analogue. In this work, the method of biolayer interferometry is used to obtain the kinetic and equilibrium parameters of interaction of insulin detemir and monomeric Aβ forms with a chain length of 40 and 42 amino acids. A model of “insulin detemir–Aβ40/42” complexes is proposed. The data obtained can be used to study the mechanisms of the effect of insulin detemir on cognitive functions of patients with Alzheimer’s disease.
Supporting agencies: the research was funded by the Russian Science Foundation, grant No. 20-74-10072.
About the Authors
M. P. Shevelyova
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Marina P. Shevelyova, Cand. Sci. (Chem.)
142290, Moscow Region, Pushchino, Institutskaya Str., 7
E. I. Deryusheva
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Evgeniya I. Deryusheva, Cand. Sci. (Phys.- Math.)
142290, Moscow Region, Pushchino, Institutskaya Str., 7
E. L. Nemashkalova
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Ekaterina L. Nemashkalova, Cand. Sci. (Biol.)
142290, Moscow Region, Pushchino, Institutskaya Str., 7
A. A. Vologzhannikova
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Alisa A. Vologzhannikova, Cand. Sci. (Biol.)
142290, Moscow Region, Pushchino, Institutskaya Str., 7
A. A. Nazipova
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Aliya A. Nazipova
142290, Moscow Region, Pushchino, Institutskaya Str., 7
E. A. Litus
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Ekaterina A. Litus, Cand. Sci. (Med.)
142290, Moscow Region, Pushchino, Institutskaya Str., 7
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Review
For citations:
Shevelyova M.P., Deryusheva E.I., Nemashkalova E.L., Vologzhannikova A.A., Nazipova A.A., Litus E.A. Interaction of β-amyloid Peptide with Insulin Detemir Studied by Biolayer Interferometry and Molecular Modelling. Journal Biomed. 2024;20(3E):233-237. (In Russ.) https://doi.org/10.33647/2713-0428-20-3E-233-237
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