Species Determination of Soviet Chinchilla Breed Rabbits by PCR of a Cytochrome b Gene Fragment Followed by Sequencing of Amplification Products
https://doi.org/10.33647/2074-5982-21-2-8-16
Abstract
The article presents data on determining the suitability of the CYTB gene for typing rabbits of the Soviet Chinchilla breed. The detection of the target site of the CYTB gene was carried out by real-time PCR using highly specific primers and an amplifier probe; the PCR product was used in the Sanger sequencing reaction. The experiment determined that the mitochondrial cytochrome b gene can act as a marker for the genetic assessment of the purity of the Soviet Chinchilla rabbit breed and the suitability of individuals for further breeding. The method of sequencing of this section of the cytochrome b gene showed its efficacy and practical significance, allowing animals to be screened over a fairly short period of time for their use in breeding and genetic work or other research purposes.
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladislav N. Karkischenko, Dr. Sci. (Med.), Prof.
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
N. V. Petrova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nataliya V. Petrova
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
V. V. Slobodenyuk
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Vladimir V. Slobodenyuk, Cand. Sci. (Biol.)
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
E. M. Koloskova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Elena M. Koloskova, Cand. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institut Village
N. A. Ledneva
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nadezhda A. Ledneva
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
M. A. Savina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Mariya A. Savina
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
E. V. Panina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Elena V. Panina, Cand. Sci. (Biol.), Assoc. Prof.
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
A. G. Berzina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Asya G. Berzina, Cand. Sci. (Biol.)
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
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Review
For citations:
Karkischenko V.N., Petrova N.V., Slobodenyuk V.V., Koloskova E.M., Ledneva N.A., Savina M.A., Panina E.V., Berzina A.G. Species Determination of Soviet Chinchilla Breed Rabbits by PCR of a Cytochrome b Gene Fragment Followed by Sequencing of Amplification Products. Journal Biomed. 2025;21(2):8-16. (In Russ.) https://doi.org/10.33647/2074-5982-21-2-8-16
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