Annotation of Minor Biologically Active Compounds in Musk Extracts of Siberian Musk Deer using High-Resolution HPLC-MS

This study presents the results of an investigation into minor biologically active compounds in extracts of Siberian musk deer (Moschus moschiferus) musk using high-performance liquid chromatography coupled with high-resolution mass spectrometry. A qualitative and semi-quantitative analysis was performed on various components, including steroids, terpenoids, peptides, and low-molecular-weight organic compounds with potential pharmacological activity. Several compounds, previously unidentified in this biological material, were detected, enhancing our understanding of the chemical composition of musk deer musk and its potential applications in biomedicine.

1. Karkischenko V.N., Dulya M.S., Ageldinov R.A., Khvostov D.V., Lyublinskiy S.L. Analiz biologicheski aktivnyh soedinenij muskusa kabargi (Moschus moschiferus) metodom gazovoj hromatografii s massselektivnym detektorom [Analysis of biologically active musk compounds of musk deer (Moschus moschiferus) by gas chromatography with mass selective detector]. Biomeditsina [Journal Biomed]. 2018;1:19–39. (In Russian).

2. Nesterov M.S., Ageldinov R.A., Khvostov D.V., Kokhan V.S., Levashova A.I., Lyublinskiy S.L., Karkischenko V.N. Standartizaciya kriticheskih pokazatelej kachestva liposom ekstrakta prepucial'noj zhelezy muskusa kabargi sibirskoj [Standardization of Critical Quality Indicators of Liposomes of Siberian Musk Deer Prepucial Gland Extract]. Biomeditsina [Journal Biomed]. 2021;17(3):62–67. (In Russian). DOI: 10.33647/2074-5982-17-3-62-67.

3. Bulbake U., Doppalapudi S., Kommineni N., Khan W. Liposomal Formulations in Clinical Use: An Updated Review. Pharmaceutics. 2017;9(2). DOI: 10.3390/pharmaceutics9020012.

4. Lv S.Q., Lei Z.X., Yan G., et al. Chemical compositions and pharmacological activities of natural musk (Moschus) and artificial musk: a review. J. of Ethnopharmacology. 2022;284:114799.

5. Wang Yu., Yang P., Chen T., Hu J., An X., Yao C., Xu L., Xu Y., Liu Sh. Analysis and comparison of blood metabolome of forest musk deer in musk secretion and non‑secretion periods. Scientific Reports. 2024;14:16980. DOI: 10.1038/s41598-024-67981-z.