On the Advantages of Humanized Transgenic Mice in Assessing Humoral Immune Response and Extrapolation to Humans
https://doi.org/10.33647/2074-5982-21-4-12-21
Abstract
Transgenic humanized mice are widely used in biomedical research for the development of new vaccines and pharmaceutical products. Specialists of the Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia have created a number of humanized mouse lines, including biomodels with an integrated human leukocyte antigen (HLA) complex. In this paper, we report a comparative analysis of the humoral immune response in mice of the HLA-A*0201 transgenic line and mice of the original line (CBA/lac × C57BL/6), F(1) hybrids) to the injected antigen – snail hemocyanin (KLH). By carrying out in vivo blood sampling from the animals of the experimental groups, the dynamics of the immune response over a period of four weeks was recorded. The titers of specific antibodies to KLH in the group of humanized mice of the HLA-A*0201 line were significantly higher than those of the original line of mice, reaching a maximum (1:51200) by the end of the experiment. The dynamics reflecting the growth of antibody synthesis as a result of repeated administration of the antigen have a similar pattern to the development of a secondary immune response in humans.
About the Authors
N. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nikolay N. Karkischenko, Dr. Sci. (Med.), Prof., Acad. of the Russian Academy of Rocket and Artillery Sciences, Corr. Member of the Russian Academy of Sciences
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
A. G. Berzina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Asya G. Berzina, Cand. Sci. (Biol.)
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
N. V. Petrova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Nataliya V. Petrova
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
E. S. Glotova
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Elena S. Glotova
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
D. V. Petrov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Dmitry V. Petrov
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
S. B. Kurashev
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Sergej B. Kurashev
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
M. A. Savina
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Mariya A. Savina
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
References
1. Gerlinskaya L.A., Maslennikova S.O., Zavyalov Ye.L., Kontsevaya G.V., Moshkin M.P. Reproduktivnii uspekh samtsov autbrednoi linii ICR pri razmnozhenii na fone antigennoi stimulyatsii [Reproductive success of outbred male ICR line when bred against antigenic stimulation]. Ontogenesis. 2012;43(5):357. (In Russian)].
2. Karkischenko V.N., Berzina A.G., Pomytkin I.A., Glotova E.S., Savina M.A., Petrov D.V., Taboyakova L.A., Bolotskikh L.A., Vasileva I.A. Izuchenie immunnogo otveta u transgennikh gumanizirovannikh mishei linii HLA-A*02:01 na vvedenie antigena — IgG loshadi [Study of the immune response in transgenic humanized HLA-A*02:01 mice to the administration of the horse IgG antigen]. Biomedicina [Journal Biomed]. 2024;20(2):45–52. (In Russian)]. DOI: 10.33647/2074-5982-20-2-45-52.
3. Karkischenko N.N., Lazarev V.N., Manuvera V.A., Bobrovskii P.A., Petrova N.V., Koloskova E.M., Glotova E.S. Printsipi sozdaniya genno-inzhenernoi konstruktsii dlya polucheniya gumanizirovannikh transgennikh mishei, nesushchikh gen HLA-C*07:02:01:01, kak proobraz innovatsionnikh transgenno-nokautnikh biomodelei [Principles of creating a genetically engineered construct to obtain humanized transgenic mice carrying the HLA-C*07:02:01:01 gene as a prototype of innovative transgenic knockout biologics]. Biomedicina [Journal Biomed]. 2024;20(1):8–20. (In Russian)]. DOI: 10.33647/2074-5982-20-1-8-20].
4. Karkischenko V.N., Petrova N.V., Savchenko E.S., Ogneva N.S., Koloskova E.M., Maksimenko S.V., Manuvera V.A., Bobrovskii P.A., Lazarev V.N. Sozdanie polnikh gibridnikh DNKkonstruktsii s genom cheloveka HLA-A*02:01:01:01 [Creation of complete hybrid DNA constructs with the human HLA-A*02:01:01:01]. Biomedicina [Journal Biomed]. 2021;17(1):10–23. (In Russian)]. DOI: 10.33647/2074-5982-17-1-10-23.
5. Kulberg A.Ya. Regulyatsiya immunnogo otveta [Regulation of the immune response]. Moscow: Meditsina Publ., 1986:106. (In Russian)].
6. Masnaya N.V. Reaktsii immunnoj i krovetvornoj sistem u mishej raznykh linij posle antigennogo i tsitostaticheskogo vozdeistviya [Reactions of the immune and hematopoietic systems in mice of different lines after antigenic and cytostatic exposure]. Diss. … Dr. Sci. (Med.). Tomsk: Nauchnoissledovatelskij institut farmakologii Tomskogo nauchnogo tsentra Sibirskogo otdeleniya RAMN, 2005:586. (In Russian)].
7. Merfi K., Uiver K. Immunobiology by Janeway. Transl. from Engl., ed. by G.A. Ignatieva, O.A. Svitich, I.N. Dyakov. Moscow: Logosfera Publ, 2020:1184. (In Russian)].
8. Stepanov V.A. Genomi, populyacii, bolezni: etnicheskaya genomika i personificirovannaya medicina [Genomes, populations, diseases: ethnic genomics and personalized medicine]. Acta Naturae (Russian version). 2010;2(4):18–34. (In Russian)].
9. Khaitov R.M., Alexeev L.P., Gudima G.O., Kofiadi I.A. Allel′nie varianty genov cheloveka, zatragivayushchie vnutrikletochniy zhiznenniy cikl VICh i reguliruyuzhie immunniy otvet na VICh-infekciyu [Allelic variants of human genes affecting the intracellular life cycle of HIV and regulating the immune response to HIV infection]. Bulletin of Siberian Medicine. 2019;18(1):119–130. (In Russian)].
10. Gray S.J., Foti S.B., Schwartz J.W., Bachaboina L., Taylor-Blake B., Coleman J., Ehlers M.D., Zylka M.J., McCown T.J., Samulski R.J. Optimizing promoters for recombinant adeno-associated virus-mediated gene ex pression in the peripheral and central nervous system using self-complementary vectors. Hum. Gene Ther. 2011;22(9):1143–1153. DOI: 10.1089/hum.2010.245.
11. Shiina T., Blancher A., Inoko H., Kulski J.K. Comparative genomics of the human, macaque and mouse major histocompatibility complex. Immunology. 2017;150:127–138. DOI: 10.1111/imm.12624.
Review
For citations:
Karkischenko N.N., Berzina A.G., Petrova N.V., Glotova E.S., Petrov D.V., Kurashev S.B., Savina M.A. On the Advantages of Humanized Transgenic Mice in Assessing Humoral Immune Response and Extrapolation to Humans. Journal Biomed. 2025;21(4):12-21. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-12-21
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