Vaccination with Recombinant Modified Myostatin Induces an Autoimmune Response to Myostatin in Calves
https://doi.org/10.33647/2074-5982-21-4-54-58
Abstract
The search for ways to increase the meat productivity of agricultural animals is an important direction in the field of immunobiotechnology. Immunization of sheep with recombinant myostatin (rMST) led to the development of an antibody response correlating with animal weight gain. The possibility of enhancing the immunogenicity of a recombinant analogue of endogenous mature myostatin for the purpose of vaccination of young cattle was considered. A genetic construct was created for the expression of recombinant modified myostatin (rmMST), in which cysteine was replaced by serine, and the producer strain E. coli BL21(DE3)/pET-mMSTN was obtained, expressing the recombinant protein with high efficiency. Calves were immunized with rMST and rmMST twice with an interval of 30 days. Using the ELISA test system, a higher immunogenicity of rmMST was shown.
Supporting agencies: the work was performed in the framework of state project No. 0445-2021-0002.
About the Authors
E. M. Koloskova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Elena M. Koloskova, Cand. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institute VillageV. A. Ezerskiy
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Vadim A. Ezerskiy
249013, Kaluga Region, Borovsk, Institute VillageK. S. Ostrenko
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Konstantin S. Ostrenko, Dr. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institute VillageN. V. Belova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Centre of Animal Husbandry — All-Russian Institute of Animal Husbandry named after Acad. L.K. Ernst
Russian Federation
Russian Federation
Nadezhda V. Belova, Cand. Sci. (Biol.)
249013, Kaluga Region, Borovsk, Institute VillageReferences
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Review
For citations:
Koloskova E.M., Ezerskiy V.A., Ostrenko K.S., Belova N.V. Vaccination with Recombinant Modified Myostatin Induces an Autoimmune Response to Myostatin in Calves. Journal Biomed. 2025;21(4):54-58. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-54-58
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