Diversity of L-methionine Sulfoximine Acetyltransferases from Clinically Important Bacterial Species of the Enterobacter
https://doi.org/10.33647/2074-5982-21-4-63-66
Abstract
L-methionine derivatives (L-methionine sulfoximine (MSX) and L-methionine sulfone (MSO)) are inhibitors of L-glutamine synthetase, which explains their toxic effect on cells. These derivatives are used in medical practice for treating inflammation, tuberculosis, and oncological diseases. Bacteria of the Salmonella, Pseudomonas, and Acinetobacter genera are resistant to the toxic effect of MSX and MSO due to the activity of L-methionine sulfoximine acetyltransferases (MSX-NAT). In this work, MSX-NAT from various Enterobacter species from the ESKAPE group of pathogens was analyzed.
An analysis of multiple enzyme alignments revealed a high percentage of their sequence identity, despite the diversity of their polypeptide chain lengths. In addition, unique insertions in the N-terminal region of the proteins were found, the functions of which remain to be clarified.
Keywords
Supporting agencies: research was funded by a grant from the Russian Science Foundation No. 23-24-00478.
About the Authors
T. A. Kudryashov
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Timofey A. Kudryashov
142290, Moscow Region, Pushchino, Nauki Ave., 3
M. V. Trunilina
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Maria V. Trunilina
142290, Moscow Region, Pushchino, Nauki Ave., 3
V. V. Bykov
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Vyacheslav V. Bykov
142290, Moscow Region, Pushchino, Nauki Ave., 3
A. S. Sokolov
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Andrey S. Sokolov, Cand. Sci. (Biol.)
142290, Moscow Region, Pushchino, Nauki Ave., 3
Yu. S. Lapteva
Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Russian Federation
Russian Federation
Lapteva Yulia Sergeevna, Cand. Sci. (Biol.)
142290, Moscow Region, Pushchino, Nauki Ave., 3
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Review
For citations:
Kudryashov T.A., Trunilina M.V., Bykov V.V., Sokolov A.S., Lapteva Yu.S. Diversity of L-methionine Sulfoximine Acetyltransferases from Clinically Important Bacterial Species of the Enterobacter. Journal Biomed. 2025;21(4):63-66. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-63-66
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