Investigation of Metabolic and Morphometric Dynamics of Human Fibroblast Spheroids
https://doi.org/10.33647/2074-5982-21-4-67-72
Abstract
Three-dimensional cellular models are known to be optimal for studying the response of tissues to external factors due to their ability to accurately replicate the physiological state of cell populations. The present study investigates the metabolic activity and morphological properties of three-dimensional cellular models (spheroids) obtained from human embryo lung fibroblasts FLEH-104 and human fibroblasts hTERT. Cellular spheroids were obtained by means of the low-adhesive surface method using the following cell concentrations: 5, 8, 10, 15, 30, 60 thousand cells/spheroid. The metabolic activity of the as-obtained spheroids was assessed using an MTT-test. The study revealed the dependence of the studied parameters of spheroids on the type of cell lines from which they were obtained, as well as on the initial concentration of cells.
About the Authors
A. K. Lapenko
Obninsk Institute for Nuclear Power Engineering — Branch of the National Research Nuclear University “MEPhI” (Moscow Engineering Physics Institute)
Russian Federation
Russian Federation
Alina K. Lapenko
249039, Kaluga Region, Obninsk, Studgorodok, 1
L. N. Komarova
Obninsk Institute for Nuclear Power Engineering — Branch of the National Research Nuclear University “MEPhI” (Moscow Engineering Physics Institute)
Russian Federation
Russian Federation
Ludmila N. Komarova, Dr. Sci. (Biol.), Prof.
249039, Kaluga Region, Obninsk, Studgorodok, 1
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Review
For citations:
Lapenko A.K., Komarova L.N. Investigation of Metabolic and Morphometric Dynamics of Human Fibroblast Spheroids. Journal Biomed. 2025;21(4):67-72. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-67-72
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