Determination of Bile Acids in Human Blood Serum by High-performance High-resolution Liquid Chromatography Mass Spectrometry
https://doi.org/10.33647/2074-5982-21-4-82-85
Abstract
This article proposes a method for measuring the mass concentrations of bile acids in human blood serum. Monitoring changes in the bile acid content is of great importance for the prevention, diagnosis, and treatment of various diseases. Selective determination of bile acids is a particularly difficult task due to their structural similarities, which complicates their chromatographic separation. Chromatographic separation parameters were selected for eight bile acids. The determination was performed using an Ultimate 3000 liquid chromatograph with a Q-Exactive mass-selective detector with electrospray ionization. The developed technique allows selective determination of eight compounds in a single analysis in a linear concentration range of 10–3000 ng/mL. In future studies, the method will be validated for other parameters.
Supporting agencies: the Russian Science Foundation (project No. 23-15-00510) supported the work
About the Authors
T. I. Alushina
Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Tatyana I. Alushina, Cand. Sci. (Chem.)
188663, Leningrad Region, Vsevolozhsky District, Kuz'molovskoje, Kuz'molovsky Urban Village, Zavodskaja Str., 6/2, Building 93
A. S. Venediktov
Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Anton S. Venediktov
188663, Leningrad Region, Vsevolozhsky District, Kuz'molovskoje, Kuz'molovsky Urban Village, Zavodskaja Str., 6/2, Building 93
References
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Review
For citations:
Alushina T.I., Venediktov A.S. Determination of Bile Acids in Human Blood Serum by High-performance High-resolution Liquid Chromatography Mass Spectrometry. Journal Biomed. 2025;21(4):82-85. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-82-85
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