Changes in the Genotoxic Properties of Etoposide when Combined with Polypore Fungus Extracts
https://doi.org/10.33647/2074-5982-21-4-105-109
Abstract
The comet assay method is used to assess DNA damage at the level of individual cells under the action of various factors, such as carcinogens, radiation and oxidative stress. In this study, we set out to evaluate the protective properties of polypore fungus extracts when combined with etoposide, an antitumor drug. The study was conducted using D. melanogaster as a model object. Larvae of the Canton-S laboratory strain were cultured on substrates with polypore fungus extracts (F. pinicola, F. fomentarius, and G. applanatum), both separately (a medium concentration of 0.5%) and in combination with etoposide (0.04%). The extracts of F. pinicola and F. fomentarius did not exhibit protective properties by enhancing the effect of the drug. Conversely, G. applanatum extract exhibited protective properties against DNA damage. The data obtained are promising for the development of new approaches to reducing the side effects of chemotherapy and increasing its effectiveness.
About the Authors
A-V. V. Vasilevskaya
Ural Federal University named after the First President of Russia B.N. Yeltsin
Russian Federation
Russian Federation
Angelika-Vladilena V. Vasilevskaya
620002, Yekaterinburg, Mira Str., 19
O. N. Antosyuk
Ural Federal University named after the First President of Russia B.N. Yeltsin
Russian Federation
Russian Federation
Olga N. Antosyuk, Cand. Sci. (Biol.), Assoc. Prof.
620002, Yekaterinburg, Mira Str., 19
V. V. Kostenko
Kazan (Volga Region) Federal University
Russian Federation
Russian Federation
Viktoria V. Kostenko, Cand. Sci. (Biol.), Assoc. Prof.
420008, Republic of Tatarstan, Kazan, Kremlevskaya Str., 18
A. A. Ermoshin
Ural Federal University named after the First President of Russia B.N. Yeltsin
Russian Federation
Russian Federation
Alexandr A. Ermoshin, Cand. Sci. (Biol.), Assoc. Prof.
620002, Yekaterinburg, Mira Str., 19
References
1. Antosyuk O., Kostenko V., Ermoshin A., Kiseleva I. Extracts from Four Species of Xylotrophic Basidiomycetes Growing in the Middle Urals (Russia) Revealed Cytotoxic Effects on Model Object Drosophila melanogaster. Int. J. of Medicinal Mushrooms. 2024;26(12):49– 61. DOI: 10.1615/IntJMedMushrooms.2024055598.
2. Lee S., Lee M., Lee K.-R., Lee E., Lee H.-J. Fomes fomentarius ethanol extract exerts inhibition of cell growth and motility induction of apoptosis via targeting AKT breast cancer MDA-MB-231 cells. Int. J. Mol. Sci. 2019;20(5):1147. DOI: 10.3390/ijms20051147.
3. Li X.-Y., Li Sh.-Y., Yin F., Chen H.-M., Yang D.-F., Liu X.-Q., Lv X.-M., Mans D., Zhang X.-D., Liang Z.-S. Antioxidative and Cytoprotective Effects of Ganoderma applanatum and Fomitopsis pinicola in PC12 Adrenal Phaeochromocytoma. Cells. 2022;24(10):15–29. DOI: 10.1615/IntJMedMushrooms.2022045050.
4. Lim G.M., Cho G.-W. Mangiferin protects mesenchymal stem cells against DNA damage and cellular aging via SIRT1 activation. Mechanisms of Ageing and Development. 2025;224:112038. DOI: 10.1016/j.mad.2025.112038.
5. Nguyen H.A.Th., Ho T.Ph., Mangelings D., Eeckhaut A.V., Heyden Y.V., Tran H.T.M. Antioxidant, neuroprotective, and neuroblastoma cells (SH-SY5Y) differentiation effects of melanins and arginine-modified melanins from Daedaleopsis tricolor and Fomes fomentarius. BMC Biotechnology. 2024;24:89.
6. Park C-G., Lim Y-B. Evaluation of antimutagenic and antioxidant properties in Fomes fomentarius L.: potential development as functional food. Appl. Sci. 2024;14(9):3927. DOI: 10.3390/app14093927.
7. Turner S.D., Wijnhoven S.W., Tinwell H., Lashford L.S., Rafferty J.A., Ashby J., Vrieling H., Fairbairn L.J. Assays to predict the genotoxicity of the chromosomal mutagen etoposide — focusing on the best assay. Mutation research/Genetic Toxicology and Environmental Mutagenesis. 2001;493(1–2):139–147. DOI: 10.1016/s1383-5718(01)00170-x.
8. Vock E.H., Lutz W.K., Hormes P., Hoffman H.D., Vamvakas S. Discrimination between genotoxicity and cytotoxicity in the induction of DNA double-strand breaks in cells treated with etoposide, melphalan, cisplatin, potassium cyanide, Triton X-100, and γ-irradiation. Mutation research/Genetic Toxicology and Environmental Mutagenesis. 1998;413(1):83–94. DOI: 10.1016/S1383-5718(98)00019-9.
9. Zhang Z., Wu Ch., Wang M., Chen J., Lv G. Chemical fingerprinting and the biological properties of extracts from Fomitopsis pinicola. Arabian Journal of Chemistry. 2023;16(5):104669. DOI: 10.1016/j.arabjc.2023.104669.
Review
For citations:
Vasilevskaya A.V., Antosyuk O.N., Kostenko V.V., Ermoshin A.A. Changes in the Genotoxic Properties of Etoposide when Combined with Polypore Fungus Extracts. Journal Biomed. 2025;21(4):105-109. (In Russ.) https://doi.org/10.33647/2074-5982-21-4-105-109
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