Simultaneous determination of clomipramine and amitriptyline in human serum using HPLC-MS / MS
Abstract
Selective, sensitive and reproducible method for the quantitative determination of amitriptyline and clomipramine in human serum by reversed-phase high performance liquid chromatography with tandem mass spectrometry (HPLC-MS / MS) using both electrospray (ESI) and chemical ionization at atmospheric pressure (APCI) was developed. Sample preparation was performed by protein precipitation with acetonitrile. Detection was performed in positive ionization mode by monitoring of multiple reactions (MRM). Quantification was performed using promethazine as internal standard. The analytical range of the method is 5-1000 ng / ml for each drug. This technique can be used for therapeutic drug monitoring.
About the Authors
T. A. Rodina
ГБУЗ «ГКБ № 23 им. Медсантруд» Департамента здравоохранения г. Москвы
Russian Federation
Russian Federation
E. S. Melnikov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
N. A. Psarev
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
A. V. Sokolov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
V. V. Arhipov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
D. L. Pozdnyakov
ГБУЗ «ГКБ № 23 им. Медсантруд» Департамента здравоохранения г. Москвы
Russian Federation
Russian Federation
V. G. Kukes
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
References
1. Акарачкова Е.С., Вершинина С.В. Синдром вегетативной дистонии или депрессия? Депрессивные расстройства в общесоматической практике // РМЖ. - 2010.- Т. 1. - № 26.
2. Кукес В.Г., Сычев Д.А., Гасанов Н.А. Проблемы клинической фармакогенетики на современном этапе // Клиническая медицина. - 2007. - Т. 85. - № 2. - C. 58-63.
3. Руководство по экспертизе лекарственных средств / под. ред. проф. А.Н. Миронова. Том I. - М.: Гриф и К, 2013. - 328 с.
4. Смулевич А.Б., Дубницкая Э.Б., Дробижев М.Ю. и др. Депрессии и возможности их лечения в общемедицинской практике (предварительные результаты программы ПАРУС) // Consilium-medicum. - 2007. - Т. 2. - № 2. - С.23-25.
5. Bromet E., Andrade L.H., Hwang I., Sampson N.A., Alonso J., de Girolamo G., de Graaf R., Demyttenaere K., Hu C., Iwata N., Karam A.N., Kaur J., Kostyuchenko S., Lepine J.-P., Levinson D., Matschinger H., Mora M.E.M., Browne M.O., Posada-Villa J., Viana M.C., Williams D.R., Kessler R.C. Cross-National Epidemiology of DSM-IV Major Depressive Episode // BMC Medicine, 2011.
6. Ciraulo D.A., Shader R.L., Greenblatt P.J. Drug interactions in psychopharmacology. In: Manual of Psychiatric therapeutic. Ed. Shader R. Little Brown and Comp (Inc.). - 1994. No. 193. - P. 8.
7. Coppen A. The biochemistry of affective disorders // Brit. J. Psych. - 1967. - No. 113. - Pp. 1237-64.
8. Guidance for Industry: Bioanalytical method validation. U.S. Department of health and human services, food and drug administration, Center for drug evolution and research (CDER). - Washington, DC: U.S. Government printing office, 2001.
9. Guideline on bioanalytical method validation, European medicines agency, committee for medicinal products for human use. - London, 2011.
10. Hall H., Orgen S.O. Effects of antidepressant drugs on different receptors in the brain // Eur. J. Pharmacol. - 1981. - No. 70. - Pp. 393-407.
11. Lapin I.P., Oxenkrug G.F. Intensification of the serotoninergic processes as a possbie determination of the thymoleptic effect // Lancet. - 1969. - No. I(7587). - Pp. 132-6.
12. Murphy P.J. The development of drug metabolism research as expressed in the publications of ASPET: Part 3, 1984-2008 // Drug metabolism and disposition. - 2008. - Vol. 36. - No. 10. - Pp. 1977-1982.
Review
For citations:
Rodina T.A., Melnikov E.S., Psarev N.A., Sokolov A.V., Arhipov V.V., Pozdnyakov D.L., Kukes V.G. Simultaneous determination of clomipramine and amitriptyline in human serum using HPLC-MS / MS. Journal Biomed. 2016;(1):43-51. (In Russ.)
Views:
271
Email this article 
