Simultaneous determination of clomipramine and amitriptyline in human serum using HPLC-MS / MS
Abstract
Selective, sensitive and reproducible method for the quantitative determination of amitriptyline and clomipramine in human serum by reversed-phase high performance liquid chromatography with tandem mass spectrometry (HPLC-MS / MS) using both electrospray (ESI) and chemical ionization at atmospheric pressure (APCI) was developed. Sample preparation was performed by protein precipitation with acetonitrile. Detection was performed in positive ionization mode by monitoring of multiple reactions (MRM). Quantification was performed using promethazine as internal standard. The analytical range of the method is 5-1000 ng / ml for each drug. This technique can be used for therapeutic drug monitoring.
About the Authors
T. A. Rodina
ГБУЗ «ГКБ № 23 им. Медсантруд» Департамента здравоохранения г. Москвы
Russian Federation
Russian Federation
E. S. Melnikov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
N. A. Psarev
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
A. V. Sokolov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
V. V. Arhipov
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
D. L. Pozdnyakov
ГБУЗ «ГКБ № 23 им. Медсантруд» Департамента здравоохранения г. Москвы
Russian Federation
Russian Federation
V. G. Kukes
ГБОУ ВПО Первый МГМУ им И.М. Сеченова Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Rodina T.A., Melnikov E.S., Psarev N.A., Sokolov A.V., Arhipov V.V., Pozdnyakov D.L., Kukes V.G. Simultaneous determination of clomipramine and amitriptyline in human serum using HPLC-MS / MS. Journal Biomed. 2016;(1):43-51. (In Russ.)
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