Specific pharmacological activity study of glycosylated polypeptide complex extracted from sea urchin Strongylocentrotus droebachiensis in the model of acute bronchitis in rats
Abstract
The anti-inflammatory activity of glycosylated polypeptide complex (GPC) extracted from sea urchin Strongylocentrotus droebachiensis was studied in the model of acute bronchitis induced by a cigarette smoke in rats. The experiment was performed in Wistar male rats. All animals were divided into six groups: four experimental, one control and one intact group. Animals in experimental groups were inhalated by GPC at 25, 50 and 100 μg/kg and also by a reference drug Ambrobene in 3.6 mg/kg, once a day, starting from the 14th day from acute bronchitis induction and during the following 14 days. Pathology induction was performed by an exposure to a cigarette smoke using special equipment. Hematological analysis, bronchopulmonary lavage cell composition and histological study of the left lung tissue at the level of the middle third (lung hilum) were used as the criteria for evaluation. GPC at all tested doses reduced the number of leukocytes in bronchoalveolar lavage fluid by 58%, and at 50 and 100 μg/kg reduced the number of leukocytes in blood by 44% compared to control group. Microscopic observation of bronchial tissue evidenced about a dose-dependent reduction of bronchi thickness after administration of GPC. Maximal thickness reduction was observed at 100 μg/kg and reached 61% comparing with control group.
About the Authors
A. E. Katelnikova
ФГБОУ ВО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Минздрава России
Russian Federation
Russian Federation
K. L. Kryshen
ЗАО «Санкт-Петербургский институт фармации»
Russian Federation
Russian Federation
M. N. Makarova
ЗАО «Санкт-Петербургский институт фармации»
Russian Federation
Russian Federation
V. G. Makarov
ЗАО «Санкт-Петербургский институт фармации»
Russian Federation
Russian Federation
V. V. Vorobieva
ФГБОУ ВО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Минздрава России
Russian Federation
Russian Federation
A. N. Shikov
ФГБОУ ВО «Северо-Западный государственный медицинский университет им. И.И. Мечникова» Минздрава России; ЗАО «Санкт-Петербургский институт фармации»
Russian Federation
Russian Federation
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Review
For citations:
Katelnikova A.E., Kryshen K.L., Makarova M.N., Makarov V.G., Vorobieva V.V., Shikov A.N. Specific pharmacological activity study of glycosylated polypeptide complex extracted from sea urchin Strongylocentrotus droebachiensis in the model of acute bronchitis in rats. Journal Biomed. 2018;(2):85-94. (In Russ.)
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