Analysis of the gene expression profile NOS2, PDE5A and HIF-1α by the digital droplet PCR

The effect of the release product of the musk glands of Moschus moschiferus was determined by determining the expression levels of the molecules of the 1α subunit of the hypoxia inducible factor, inducible nitric oxide synthase and cyclic guanosine monophosphate -specific phosphodiesterase type 5A in experimental animals under stress caused by forced activity. The biological materials (brain, heart and lungs) of Wistar rats that received the substance under study and undergone a forced swim test with a cargo were examined in the work. The gene expression profile was determined by droplet digital polymerase chain reaction. Against the background of the introduction of test substances, the expression levels of the studied are characterized by the expected values, which demonstrate the accelerated start of multifactorial mechanisms of adaptation of the organism and amplifies the ability of tissues to experience unfavorable conditions upon administration of active components of the musk glands of Moschus moschiferus .

Siberian musk Moschus moschiferus, droplet digital polymerase chain reaction, subunit 1α of the hypoxia inducible factor, inducible nitric oxide synthase, cyclic guanosine monophosphate - specific phosphodiesterase type 5A, expression

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