Antagonistic activity of a consortium of strains Lactobacillus fermentum ВКМ В-2793D and Lactobacillus reuteri ВКМ В-3144D isolated from human milk to mastitis-inductioning pathogens

The aim of the study was to study the antagonistic activity of strains of Lactobacillus fermentum BKM B-2793D, Lactobacillus reuteri BKM B-3144D isolated from human breast milk and their consortium to pathogens that cause mastitis during the lactation period. The lactobacilli and their consortium reveal antimicrobial activity against the indicator microorganisms used in this study including pathogens resistant to antibiotics. The consortium protects human mammary gland MCF-7 cells against adhesion and colonization by Staphylococcus aureus 8325-4. The obtained results suggest that lactobacilli from breast milk of healthy woman L. fermentum ВКМ В-2793D and L. reuteri ВКМ В-3144D and their consortium are excellent candidates for the development of probiotic preparation for prophylactics and treatment of lactational mastitis.

lactobacilli of human milk, antagonistic activity, biomodelling

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26. Delgado S., Garcia P., Fernandez L., Jimenez E., Rodriguez-Banos M., Del Campo R., Rodriguez J.M. Characterization of Staphylococcus aureus strains involved in human and bovine mastitis. FEMS immunological and medical microbiology. 2011. V. 62. Pp. 225-235.

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28. Indrio F., Neu J. The intestinal microbiome of infants and the use of probiotics. Cur. Opin. Pediatr. 2011. V. 23. No. 2. Pp. 145-150.

29. Jost T., Lacroix C., Braegger C., Rochat F., Chassart C. Vertical mother-neonate transfer of maternal gut bacteria via breast-feeding. Environmental Microbiology. 2013. doi:10.1111/1462-2920.12238.

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31. Lehri B., Seddon A., Karlyshev A. Potential probiotic-associated traits revealed from completed high quality genome sequence of Lacobacillus fermentum 3872. Standards in Genomic Sciences. 2017. doi:10.1186/s40793-017-0228-4.

32. Lehri B., Seddon A., Karlyshev A. Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity. FEMS Microbiol. Let. 2015. V. 362. Pp. 1-5.

33. Martin V., Maldonado A., Moles L., et al. Sharing of bacterial strains between breast milk and infant feces. J. of Human Lactation. 2012. V. 28. Pp. 36-44.

34. Ramiah K., van Reenen C., Dicks L. Surface-bound proteins of Lactobacillus plantarum 423 that contribute to adhesion of Caco-2 cells and their role in competitive exclusion and displacement of Clostridium sporogenes and Enterococcus faecalis. Res. Microbiol. 2008. V. 6. Pp. 470-475.

35. Saiman L., Okeefe M., Graham P., Wu F., et al. Hospital transmission of community-acquired methicillin-resistant Staphylococcus aureus among postpartum women. Clin. Infect. Dis. 2003. V. 37. Pp. 1313-19.

36. Schoenfelder S.M., Lange С., Eckart M., Hennig S., Kozytska S., Ziebuhr W. Successthrough diversity - how Staphylococcus epidermidis establishes as a nosocomial pathogen. Int. J. of Medical Microbiology. 2010. V. 300. Pp. 380-386.

37. Sears P.M., McCarthy K.K. Diagnosis of mastitis for therapy decisions. Veterinary Clinics of North America: Food Animal Practice. 2003. V. 19. Pp. 93-108.

38. Soto A., Martín V., Jiménez E., Mader I., Rodríguez J.M., Fernández L. Lactobacilli and bifidobacteria in human breast milk: influence of antibiotherapy and other host and clinical factors. J. Pediatr. Gastroenterol. Nutr. 2014. V. 59. Pp. 78-88.

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40. World Health Organization. Mastitis: causes and management. Geneva: WHO, 2000.