Proteomic analysis in the identification of active components in the preputial gland secretion of the siberian musk deer
https://doi.org/10.33647/2074-5982-15-1-35-47
Abstract
The Siberian musk deer (Moschus Moschiferus) is a deer-like animal. The substance that is secreted by sexually mature males is highly valued worldwide for its perceived pharmaceutical and perfumery properties. However, there is a lack of comprehensive information regarding the component composition of the musk and tissues of this species. A complex study of the protein composition of biologically active components in the preputial gland secretion of the Siberian musk deer was performed using the methods of size-exclusion chromatography and peptide mapping. The latter method was realized using high-performance liquid chromatography tandem mass-spectrometric detection following fermentation by trypsin. These are effective methods for generating vast amounts of data that can facilitate research on the Siberian musk deer. A molecular weight distribution characteristic of the investigated extracts was established. Optimal conditions for the extraction, chromatographic separation and relative quantitative determination of the main musk components were determined. Detailed information on the most significant (major and minor) protein components in the studied samples is presented in accordance with the algorithm of the Spectrum Mill MS Proteomics Workbench search program and the UniProt protein database. Protein profiling data was clustered according to their molecular and biological functions. The identified proteins were assessed in terms of possible mechanisms of biological action and targets, which can be affected by the protein components of the objects under the study. A conclusion is made about a multicomponent composition of the Siberian musk deer preputial gland secretion. Marker protein components in the studied extracts are suggested. Possible links between the identified proteins and biological effects are outlined.
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical Biological Agency of Russia
Russian Federation
Russian Federation
Dr. Sci. (Med.), Prof.,
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
M. S. Dulya
Scientific Center of Biomedical Technologies of the Federal Medical Biological Agency of Russia
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
D. V. Khvostov
Scientific Center of Biomedical Technologies of the Federal Medical Biological Agency of Russia
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
R. A. Ageldinov
Scientific Center of Biomedical Technologies of the Federal Medical Biological Agency of Russia
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
Russian Federation
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
S. L. Lyublinskiy
Scientific Center of Biomedical Technologies of the Federal Medical Biological Agency of Russia
Russian Federation
Russian Federation
PhD Sci. (Biol.),
143442, Moscow region, Krasnogorsk, Setllement Svetlye Gory, building 1
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Review
For citations:
Karkischenko V.N., Dulya M.S., Khvostov D.V., Ageldinov R.A., Lyublinskiy S.L. Proteomic analysis in the identification of active components in the preputial gland secretion of the siberian musk deer. Journal Biomed. 2019;(1):35-47. (In Russ.) https://doi.org/10.33647/2074-5982-15-1-35-47
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