Impact of Chronic Formalin-Induced Inflammation on Tumour Development and Longevity in White Outbred Rats
https://doi.org/10.33647/2074-5982-17-1-82-93
Abstract
The work aimed to investigate the impact of chronic formalin-induced inflammation on the remote malignancy development and longevity in white outbred tumour-bearing rats.
Experiments were conducted with 29 white outbred mature male rats divided in three groups: a control, water-injection and main formalin-injection group. Aqueous formaldehyde or same water volume were injected into left ankle joint thrice prior to Guerin’s carcinoma transplantation, 4 weeks past the first injection. The tumour size dynamics, longevity, oedema severity, blood leucocyte count and overall motor activity were estimated with an Open Field test. Statistical analysis was performed with Statistica 6 using the Kruskal—Wallis and Dunn’s test criteria.
Formalin administration accelerated the tumour growth in most animals. Nevertheless, longevity increased relative to the control’s maximum in 50% cases. Lethality in such cases was often registered with larger tumours relative to the control group. The pre-tumour traits of inflammatory response and motor dynamics in longer-lived animals have been revealed. A similar trend was registered in water administration trials at a less pronounced inflammatory and algetic response relative to the formalin trials. Putative chronic inflammation-associated mechanisms of anti-tumour resistance have been proposed.
A multidirectional effect of formalin-induced inflammation on tumourigenesis is shown for the first time: a prevailing tumour growth acceleration at higher longevity with seldom tumour inhibition cases in white outbred male rats. The mechanisms of inflammation-associated anti-tumour resistance require further research. The results relate to the known effect of musculoskeletal inflammatory conditions on tumourigenesis.
About the Authors
G. V. ZhukovaRussian Federation
Galina V. Zhukova - Dr. Sci. (Biol.)
344037, Rostov-on-Don, 14 Liniya Street, 63
A. I. Shikhlyarova
Russian Federation
Alla I. Shikhliarova - Dr. Sci. (Biol.), Prof.
344037, Rostov-on-Don, 14 Liniya Street, 63
T. P. Protasova
Russian Federation
Tatiana P. Protasova - Cand. Sci. (Biol.)
344037, Rostov-on-Don, 14 Liniya Street, 63
E. A. Lukbanova
Russian Federation
Ekaterina A. Lukbanova
344037, Rostov-on-Don, 14 Liniya Street, 63
E. V. Zaikina
Russian Federation
Ekaterina V. Zaikina
344037, Rostov-on-Don, 14 Liniya Street, 63
А. V. Volkova
Russian Federation
Anastasiya V. Volkova
344037, Rostov-on-Don, 14 Liniya Street, 63
D. V. Khodakova
Russian Federation
Darya V. Khodakova
344037, Rostov-on-Don, 14 Liniya Street, 63
References
1. Glantz S. Mediko-biologicheskaya statistika [Primer of Biostatistics]. 4th Ed. English transl. Moscow: Praktika Publ., 1998:459. (In Russian). DOI: 10.1002/(SICI)1097-0258(19990915/30)18:17/18%3C2548::AID-SIM164%3E3.0.CO;2-A.
2. Kit O.I., Franciyanc E.M., Kotieva I.M., Kaplieva I.V., Trepitaki L.K., Bandovkina V.A., et al. Nekotoryye mekhanizmy povysheniya zlokachestven-nosti melanomy na fone khronicheskoy boli u samok myshey [Some mechanisms of increasing malignancy of B16/F10 melanoma in female mice with chronic pain]. Rossiyskiy zhurnal boli [Russian J. of Pain]. 2017;53(2):14 20. (In Russian).
3. Kzhyshkovska Yu.G., Mitrofanova I.V., Zavyalova M.V., Slonimskaya E.M., Cherdyntseva N.V. Opuholeassociirovannye makrofagi [Tumor associated macrophages]. Moscow: Nauka Publ., 2017:224. (In Russian).
4. Kong Kh.Kh., Khaziakhmetova V.N., Ziganshina L.E. Novyy metod sravneniya effektivnosti NPVS na eks-perimentalnykh modelyakh vospaleniya pri dlitelnom nablyudenii [New Approach to Comparing NSAID Efficacy by Long-Term Monitoring of Experimental Inflammation Models]. Experimental and Clinical Pharmacology. 2016;79(11):19-25. (In Russian). DOI: 10.30906/0869-2092-2016-79-11-19-25.
5. Lakin G.F. Biometrics. Moscow: Vysshaya shkola Publ., 1990:352. (In Russian).
6. Luchikhina L.V., Mendel O.I., Mendel V., Golukhov G.N. Osteoartrit i voz-rast. Rol stareniya v etiologii i patogeneze zabolevaniya [Osteoarthritis and age. Role of aging in the etiology and pathogenesis of the disease]. Sovremennaya revmatologiya [Modern Rheumatology J.]. 2017;11(1):4-11. (In Russian). DOI: 10.14412/1996-7012-2017-1-4-11.
7. Samedov V.Kh., Naleskina L.A., Zakharychev V.D. Morfologicheskiye izmeneniya v kartsinome Gerena posle kriodestruktsii. lokalnoy SVCh-gipertermii i ikh kombinatsii [Morphological changes during Geren carcinoma after cryoblation, a local microwave hyperthermia and their combination]. Russian J. of Oncology. 2013;18(3):17-21. (In Russian).
8. Antonelli M., Kushner I. It’s time to redefine inflammation. FASEB J. 2017;31(5):1787-1791. DOI:10.1096/fj.201601326R.
9. Chovatiya R., Medzhitov R. Stress, Inflammation, and Defense of Homeostasis. Mol. Cell. 2014;54(2):281-288. DOI: https://doi.org/10.1016/j.molcel.2014.03.030.
10. Flamant M., Roblin X. Inflammatory bowel disease: towards a personalized medicine. Therap. Adv. Gastroenterol. 2018;11:1-15. DOI: 10.1177/1756283X17745029.
11. Grabovskaya S.V., Salyha Y.T. Do Results of the Open Field Test Depend on the Arena Shape? Neurophysiology. 2014;46(4):376-380. DOI: 10.1007/s11062-014-9458-x.
12. Knight B.E., Kozlowski N., Havelin J., King T., Crocker S.J., Young E.E., et al. TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms. Front. Mol. Neurosci. 2019;12:220. DOI: 10.3389/fnmol.2019.00220.
13. Korniluk A., Koper O., Kemona H., Dymicka-Piekarska V. From inflammation to cancer. Ir. J. Med. Sci. 2017;186:57-62. DOI: 10.1007/s11845-016-1464-1470.
14. Mescher A.L. Macrophages and fibroblasts during inflammation and tissue repair in models of organ regeneration. Regeneration (Oxf). 2017;4(2):39-53. DOI:10.1002/reg2.77.
15. Shinko D., Diakos C.I., Clarke S.J., Charles K.A. Cancer-Related Systemic Inflammation: The Challenges and Therapeutic Opportunities for Personalized Medicine. Clin. Pharmacol. Ther. 2017;102(4):599-610. DOI:10.1002/cpt.789.
16. Su Y.S., Sun W.H., Chen C.C. Molecular mechanism of inflammatory pain. World J. Anesthesiol. 2014;3(1):71-81. DOI: 10.5313/wja.v3.i1.71.
17. Zhukova G.V., Shikhliarova A.I., Barteneva T.A., Goroshinskaya I.A., Gudtskova T.N., Bragina M.I., et al. Some approaches to the activation of antitumor resistance mechanisms and functional analogs in the categories of synergetics. Biophysics. 2016;61(2):303-315. DOI: 10.1134/S0006350916020251.
Review
For citations:
Zhukova G.V., Shikhlyarova A.I., Protasova T.P., Lukbanova E.A., Zaikina E.V., Volkova А.V., Khodakova D.V. Impact of Chronic Formalin-Induced Inflammation on Tumour Development and Longevity in White Outbred Rats. Journal Biomed. 2021;17(1):82-93. (In Russ.) https://doi.org/10.33647/2074-5982-17-1-82-93