Assessment of nuclear material elimination by different methods of dermis decellularization
https://doi.org/10.33647/2713-0428-17-3E-59-63
Abstract
We carry out a comparative assessment of the degree of nuclear material disintegration and elimination in the samples of decellularized porcine dermis after using chemical, detergent and enzymatic decellularization methods. Decellularized dermis materials are promising materials as wound dressings. The chemical method (Protocol No. 1) was performed using NaOH and H2 O2 solutions; the detergent method (Protocol No. 2) involved the solutions of Triton X-100 and sodium deoxycholate in combination with Na2 -EDTA; the enzymatic method (Protocol No. 3) was based on the solutions of trypsin Versene and porcine pan creatic DNase. Subsequently, we analyzed the DNA amount in decellularized and native dermis (control group) samples. The results of this analysis showed positive results in all three protocols. It was found that the detergent method have advantages over other methods of producing decellularized dermis matrices.
About the Authors
K. I. Melkonyan
Kuban State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
Cand. Sci. (Med.),
350063, Krasnodar, Mitrofana Sedina Str., 4
T. V. Rusinova
Kuban State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
350063, Krasnodar, Mitrofana Sedina Str., 4
Ya. A. Kozmai
Kuban State Medical University of the Ministry of Health Сare of Russia
Russian Federation
Russian Federation
350063, Krasnodar, Mitrofana Sedina Str., 4
A. C. Asyakina
Kuban State Medical University of the Ministry of Health Сare of Russia;
Kuban State University
Russian Federation
Russian Federation
350063, Krasnodar, Mitrofana Sedina Str., 4;
350040, Krasnodar, Stavropolskaya Str., 149
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Review
For citations:
Melkonyan K.I., Rusinova T.V., Kozmai Ya.A., Asyakina A.C. Assessment of nuclear material elimination by different methods of dermis decellularization. Journal Biomed. 2021;17(3E):59-63. (In Russ.) https://doi.org/10.33647/2713-0428-17-3E-59-63
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