Comparison of methods of surgical transplantation of mice embryos into pseudopregnant female recipients
https://doi.org/10.33647/2713-0428-17-3E-80-88
Abstract
There are a lot of factors that are able to influence the result of embryo transfer to pseudopregnant females, including the transplantation procedure itself. To determine the possible role of the surgical transplantation method on the embryo transfer result, we analyzed the data of transplantation of 6715 microinjected embryos into the oviduct of 471 pseudopregnant female by three ways — into the infundibulum, into the ampullae by the oviduct muscular wall puncture or incision. A significant increase in the pregnancy rate of recipients, as well as the birth rate in total recipients in oviduct puncture/incision groups, has been shown, however, the birth rate in pregnant recipients does not differ between all experimental groups. Survival of pups is also significantly higher in oviduct puncture/incision groups.
About the Authors
E. S. Savchenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
N. S. Ogneva
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
S. V. Maksimenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
143442, Moscow Region, Krasnogorsk District, Svetlye Gory Village, 1
O. B. Zhukova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Center of Animal Husbandry — The All-Russian Institute of Animal Husbandry named after academician L.K. Ernst
Russian Federation
Russian Federation
249013, Kaluga Region, Borovsk, Institut Village
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Review
For citations:
Savchenko E.S., Ogneva N.S., Maksimenko S.V., Zhukova O.B. Comparison of methods of surgical transplantation of mice embryos into pseudopregnant female recipients. Journal Biomed. 2021;17(3E):80-88. (In Russ.) https://doi.org/10.33647/2713-0428-17-3E-80-88
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