Preparation of Bacterial Recombinant Myostatin to Induce the Synthesis of Myostatin-Specific Autoantibodies
https://doi.org/10.33647/2074-5982-18-3-22-26
Abstract
The myostatin protein, belonging to the family of growth factors, represents a potential target for therapeutic effects in muscular system pathologies. However, this protein is characterized by other beneficial properties. Polymorphisms of the myostatin gene associated with the restriction of its functional activity are useful as genetic markers of meat productivity in farm animals. Blocking the action of myostatin in productive animals can be achieved by inducing the synthesis of specific autoantibodies using recombinant myostatin, possessing sufficient immunogenicity against myostatin as an antigen. A genetic construct was created and an E. coli producer strain with a high level of expression of recombinant myostatin was obtained.
About the Authors
E. M. Koloskova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Center of Animal Husbandry — The All-Russian Institute of Animal Husbandry named after Academician L.K. Ernst
Russian Federation
Russian Federation
Elena M. Koloskova - Cand. Sci. (Biol.), AllRussian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Center of Animal Husbandry — The AllRussian Institute of Animal Husbandry named after Academician L.K. Ernst.
249013, Kaluga region, Borovsk, Institut Village.
V. A. Ezerskiy
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Center of Animal Husbandry — The All-Russian Institute of Animal Husbandry named after Academician L.K. Ernst
Russian Federation
Russian Federation
Vadim A. Ezerskiy.
249013, Kaluga region, Borovsk, Institut Village.
O. B. Zhukova
All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition — Branch of the Federal Scientific Center of Animal Husbandry — The All-Russian Institute of Animal Husbandry named after Academician L.K. Ernst
Russian Federation
Russian Federation
Olga B. Zhukova.
249013, Kaluga region, Borovsk, Institut Village.
References
1. Kukes V.G., Gazdanova A.A., Furalyov V.A., Marinin V.F., Per-kov A.V., Lenkova N.I., Solovieva S.A., Ryazantseva O.V. Sovremennoe predstavlenie o biologicheskoy roli i klinicheskom znachenii miostatina — glavnogo regulyatora rosta i differentsirovki myshts [Modern conception of myostatin biological role and clinical significance as the main regulator of muscle growth and differentiation]. Medicinskij vestnik Severnogo Kavkaza [Medical News of North Caucasus]. 2021;16(3):327-332. (In Russian). DOI: 10.14300/mnnc.2021.16079.
2. Bongiorni S., Valentini A., Chillemi G. Structural and dynamic characterization of the C313Y mutation in myostatin dimeric protein, responsible for the “double muscle” phenotype in Piedmontese cattle. Front. Genet. 2016;7:14. DOI: 10.3389/fgene.2016.00014.
3. Chen M.M., Zhao Y.P., Zhao Y., Deng S.L., Yu K. Regulation of myostatin on the growth and development of skeletal muscle. Front. Cell Dev. Biol. 2021;9:785712. DOI: 10.3389/fcell.2021.785712.
4. McPherron A.C., Lawler A.M., Lee S.J. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 1997;387(6628):83-90. DOI: 10.1038/387083a0.
5. Nielsen T.L., Vissing J., Krag T.O. Antimyostatin treatment in health and disease: The story of great expectations and limited success. Cells. 2021;10(3):533. DOI: 10.3390/cells10030533.
Review
For citations:
Koloskova E.M., Ezerskiy V.A., Zhukova O.B. Preparation of Bacterial Recombinant Myostatin to Induce the Synthesis of Myostatin-Specific Autoantibodies. Journal Biomed. 2022;18(3):22-26. (In Russ.) https://doi.org/10.33647/2074-5982-18-3-22-26
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