Dynamics of Changes in Acetylcholinesterase Activity in the Brain and Bood of Rats During Pharmacological Correction of Seizures Caused by Carbamate
https://doi.org/10.33647/2074-5982-18-3-27-31
Abstract
To simulate a convulsive syndrome, phenylcarbamate was administered intraperitoneally at a dose of 1 mg/kg. Valproic acid aminoester (43 mg/kg), caramiphene (50 mg/kg), and diferidine (2 mg/kg) were investigated as potential anticonvulsant drugs. Blood and brain tissue for the determination of acetylcholinesterase (AСhE) were taken at 10, 30, 60 minutes, 6 and 24 hours after administration of the xenobiotic. In case of poisoning with reversible inhibitors of AChE and accumulation of acetylcholine in the CNS synapses (due to inhibition of AChE activity), only the initial manifestations of convulsive activity are caused, and other mechanisms not associated with AChE inhibition are responsible for the processes of further maintenance and recurrence of seizures.
About the Authors
A. S. Melekhova
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Aleksandra S. Melekhova.
192019, Saint Petersburg, Bekhtereva Str., 1.
L. G. Kubarskaya
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Larisa G. Kubarskaya - Cand. Sci. (Biol.), Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia.
192019, Saint Petersburg, Bekhtereva Str., 1.
A. N. Petrov
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Aleksandr N. Petrov - Dr. Sci. (Med.), Prof., Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia.
192019, Saint Petersburg, Bekhtereva Str., 1.
A. Ya. Bespalov
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Aleksandr Ya. Bespalov - Cand. Sci. (Chem.), Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia.
192019, Saint Petersburg, Bekhtereva Str., 1.
A. B. Verveda
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Aleksej B. Verveda - Cand. Sci. (Med.), Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia.
192019, Saint Petersburg, Bekhtereva Str., 1.
A. V. Belskaya
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Alisa V. Belskaya.
192019, Saint Petersburg, Bekhtereva Str., 1.
M. V. Melnikova
Golikov Research Clinical Center of Toxicology of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Margarita V. Melnikova.
192019, Saint Petersburg, Bekhtereva Str., 1.
References
1. Ramsh S.M., Petrov A.N. Podkhody k ratsional'nomu konstruirovaniyu obratimykh ingibitorov atsetilkholinesterazy v kachestve sredstv dlya lecheniya bolezni Al'tsgeymera [Approaches to the rational design of reversible acetylcholinesterase inhibitors as agents for the treatment of Alzheimer's disease]. St. Petersburg; 1999. (In Russian).
2. Ellmann G.L., Courtney K.D., Andress V., Fcather-stone R.M. A new and rapid Colorimetric determination of activity acetylcholinesterase. Biochem. Pharmacol. 1961;7(2):88-95.
3. Figueiredo T.H., Aroniadou-Anderjaska V., Neuroprotective efficacy of caramiphen against soman and mechanisms of its action. Br. J. of Farmacology. 2011;164:1495-1505.
4. Principles of Ecotoxicology. Ed. Walker C.H., et al. London, New York: Taylor& Fransic Group., Boca Raton; 2006.
5. Racine R.J. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr. Clin. Neurophysiol. 1972;32(3):281-294.
Review
For citations:
Melekhova A.S., Kubarskaya L.G., Petrov A.N., Bespalov A.Ya., Verveda A.B., Belskaya A.V., Melnikova M.V. Dynamics of Changes in Acetylcholinesterase Activity in the Brain and Bood of Rats During Pharmacological Correction of Seizures Caused by Carbamate. Journal Biomed. 2022;18(3):27-31. (In Russ.) https://doi.org/10.33647/2074-5982-18-3-27-31
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