OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN
https://doi.org/10.33647/2074-5982-15-3-49-58
Abstract
Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.
About the Authors
I. L. Kotovich
Belarusian State Medical University
Belarus
Belarus
Cand. Sci. (Med.), Assoc. Prof.,
220116, Minsk, Dzerzhinskogo avenue, 83
Zh. A. Rutkovskaya
Belarusian State Medical University
Belarus
Belarus
Cand. Sci. (Med.), Assoc. Prof.,
220116, Minsk, Dzerzhinskogo avenue, 83
A. D. Taganovich
Belarusian State Medical University
Belarus
Belarus
Dr. Sci. (Med.), Prof.,
220116, Minsk, Dzerzhinskogo avenue, 83
References
1. Volchegorskij I.A., Nalimov A.G., Yarovinskij B.G., Lifshits R.I. Sopostavlenije razlichnyh podhodov k opredeleniyu produktov perekisnogo okislenija lipidov v heptan-isopropanol’nyh ekstraktah krovi [Comparison of different approaches to the determination of lipid peroxidation products in heptane-isopropanol blood extracts]. Voprosy medicinskoj himii [Questions of Medical Chemistry]. 1989;35(1):127–135. (In Russian).
2. Goncharenko M.S., Latinova A.M. Metod otsenki perekisnogo okisleniya lipidov [Method of lipid peroxidation assessment]. Laboratornoje delo [Laboratory Work]. 1985;1:60–61. (In Russian).
3. Dubinina E.E. Produkty metabolizma kisloroda v funkcionalnoj aktivnosti kletok. Zhizn’ i smert’, sozidanie i razrushenie [Oxygen metabolism products in the functional activity of cells. Life and death, creation and destruction]. Saint Petersburg: Medicinskaya pressa, 2006. 400 p. (In Russian).
4. Kotovich I.L., Rutkovskaya Zh.A., Taganovich A.D. Korrekciya oksidantno-antioksidantnogo balansa v ljogkih pri giperoksii s ispolzovaniyem liposomnyh form alfa-tokoferola i retinoidov v eksperimente [Correction of oxidant-antioxidant balance in lungs during hyperoxia using liposomal alpha-tocoferol and retinoids in the experiment]. Biomedicinskaya himiya [Biomedical Chemistry]. 2017;63(4):289–295. (In Russian).
5. Mamontova N.S., Beloborodova E.I., Tyukalova L.I. Aktivnost katalazy pri hronicheskom alkogolizme [Catalase activity in chronic alcoholism]. Klinicheskaya laboratornaya diagnostika [Clinical Laboratory Diagnostics]. 1994;1:27–28. (In Russian).
6. Moin V.M. Prostoj i specifi cheskij metod opredeleniya aktivnosti glutatioperoksidazy v eritrocitah [A simple and specifi c method for determining glutathione peroxidase activity in erythrocytes]. Laboratornoje delo [Laboratory Work]. 1986;12:724–727. (In Russian).
7. Ovsyannikov D.Yu., Petruk N.I., Kuz’menko L.G. Bronholegochnaya displaziya u detej [Bronchopulmonary dysplasia in children]. Pediatriya [Pediatrics]. 2004;1:3–8. (In Russian).
8. Ahola T., Lapatto R., Raivio K.O., Selander B., Stigson L., Jonsson B., et al. N-acetylcysteine does not prevent bronchopulmonary dysplasia in immature infants: a randomized controlled trial. J. Pediatr. 2003;143(6):713–719.
9. Chang L.-Y., Subramaniam M., Yoder B.A., Day B.J., Ellison M.C., Sunday M.E., et al. A catalytic antioxidant attenuates alveolar structural remodeling in bronchopulmonary dysplasia. Am. J. Respir. Crit. Care Med. 2003;167:57–64.
10. Davis J.M., Parad R.B., Michele T., Allred E., Price A., Rosenfeld W. Pulmonary outcome at 1 year corrected age in premature infants treated at birth with recombinant human CuZn superoxide dismutase. Pediatrics. 2003;111(3):469–476.
11. Dua J.S., Rana A.C., Bhandari A.K. Liposome: methods of preparation and applications. Int. J. Pharm. Studies and Res. 2012;3:14–20.
12. Haddad M.E., Jean E., Turki A., Hugon G., Vernus B., Bonnieu A., et al. Glutathione peroxidase 3, a new retinoid target gene, is crucial for human skeletal muscle precursor cell survival. J. Cell Science. 2012;125:6147–6156.
13. Kagan V., Bakalova R., Zhelev Z., Rangelova D., Serbinova E., Tyurin V., et al. Intermembrane transfer and antioxidant action of alpha-tocopherol in liposomes. Arch. Biochem. Biophys. 1990;280:147–152.
14. Nagata K., Iwasaki Y., Yamada T., Yuba T., Kono K., Hosogi S., et al. Overexpression of manganese superoxide dismutase by N-acetylcysteine in hyperoxic lung injury. Respir. Med. 2007;101(4):800–807.
15. Oliveira M.R. Vitamin A and retinoids as mitochondrial toxicants. Oxidative Medicine and Cellular Longevity. 2015. Article ID 140267. DOI: 10.1155/2015/140267.
16. Spears K., Cheney C., Zerzan J. Low plasma retinol concentrations increase the risk of developing bronchopulmonary dysplasia and long-term respiratory disability in very-low-birth-weight infants. Am. J. Clin. Nutr. 2004;80:1589–1594.
17. Suntres Z.E. Liposomal antioxidants for protection against oxidant-induced damage. J. Toxicol. 2011:16. ID 152474.
18. Thomas D.A., Myers M.A., Wichert B., Schreier H., Gonzalez-Rothi R.J. Acute effects of liposome aerosol inhalation on pulmonary function in healthy human volunteers Chest. 1991;99(5):1268–1270.
19. Tyson J.E., Wright L.L., Oh W., Kennedy K.A., Mele L., Ehrenkranz R.A., et al. Vitamin A supplementation for extremely-low-birth-weight infants. National Institute of child health and human development neonatal research network. New Engl. J. Med. 1999;340(25):1962–1968.
20. Watts J.L., Milner R., Zipursky A., Paes B., Ling E., Gill G., et al. Failure of supplementation with vitamin E to prevent bronchopulmonary dysplasia in infants less than 1500 g birth weight. Eur. Resp. J. 1991;4(2):188–190.
21. Wolfson M., Shinvwell E.S., Zvillich M., Rager-Zisman B. Inhibitory effect of retinoic acid on the respiratory burst of adult and cord blood neutrophils and macrophages: potential implication to bronchopulmonary dysplasia. Clin. Exp. Immunol. 1988;72:505–509.
Review
For citations:
Kotovich I.L., Rutkovskaya Zh.A., Taganovich A.D. OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN. Journal Biomed. 2019;(3):49-58. (In Russ.) https://doi.org/10.33647/2074-5982-15-3-49-58
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