A LIPOSOMAL COMPOSITION OF MUSC DEER PREPUTIAL GLAND EXTRACT AS A NEW AGENT OF ADAPTOGENIC ACTION
https://doi.org/10.33647/2074-5982-15-4-34-45
Abstract
For the first time, a liposomal form of a new original drug based on the Siberian musk deer preputial gland extract was obtained and characterized. An effective and scalable method of high-pressure homogenization was used for preparative extraction of liposomes from musk extracts. For the obtained liposomal product, such indicators as the quality of size distribution, homogeneity and the degree of inclusion of biologically active components were characterized using the methods of dynamic light scattering, transmission microscopy, preparative and analytical chromatography and chromatography-mass spectrometry. A homogeneous dispersion of musk liposomes with a uniform size distribution was obtained, with the maximum distribution values being achieved at 50 and 240 nm. The ζ-potential of the obtained nanoparticles of –35...–47 mV confirmed a high physicochemical stability of the developed liposomal dispersion. According to the gel filtration chromatography and mass spectrometry results, the degree of inclusion of the target musk extract components in the liposomes was 55–75% in terms of steroid and total protein values. The obtained quality indicators indicate that the developed liposomal composition of musk deer extracts can be used as a high-effective natural adaptogen.
Keywords
musk deer,
musk,
gland,
liposomes,
chromatography,
mass spectrometry,
adaptogens,
peptides,
proteins,
androsteroids
About the Authors
V. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Dr. Sci. (Med.), Prof.,
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
M. S. Dulya
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
Russian Federation
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
R. A. Ageldinov
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
Russian Federation
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
S. L. Lyublinskiy
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Cand. Sci. (Biol.),
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
N. N. Karkischenko
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
Russian Federation
Russian Federation
Dr. Sci. (Med.), Prof., Corresponding Member of the Russian Academy of Sciences, Academician of the Russian Academy of Rocket and Artillery Sciences,
143442, Moscow region, Krasnogorsk district, Svetlye gory village, building 1
References
1. Vinogradov V.M., Bobkov Yu.G. Farmakologicheskaya strategiya adaptacii Farmakologicheskaya regulyaciya sostoyanij dezadaptacii [Pharmacological adaptation strategy. Pharmacological regulation of maladaptation states]. Moscow: B.I., 1986. P. 3–11. (In Russian).
2. Voskresenskiy O.M. O svyazyah adaptogennogo i antioksidantnogo dejstviya. Adaptatsiya i adaptogeny [On the links of adaptogenic and antioxidant effects. Adaptation and adaptogens]. Vladivostok, 1977. P. 91–96. (In Russian).
3. Dudnichenko A.V., Krasnopol’- skiy Yu.M., Shvets V.I. Liposomal’nye lekarstvennye preparaty v eksperimente i klinike [Liposomal drugs in experiment and clinic]. Kharkov: RA-Karavella Publ, 2001. (In Russian).
4. Karkischenko V.N., Dulya M.S., Khvostov D.V., Ageldinov R.A. Analiz biologicheski aktivnyh soedinenij muskusa kabargi Moschus moschiferus metodom gazovoj hromatografi i s mass-selektivnym detektorom [The analysis of biologically active compounds of musk of Muschus moschiferus by gas chromatography with a mass-selective detector. Biomedicine. 2018;1:19–39. (In Russian).
5. Karkischenko N.N., Ujba V.V., Karkischenko V.N., Shustov E.B., Kotenko K.V., Lyublinskij S.L. Ocherki sportivnoj farmakologii. T. 3. Vektory farmakoregulirovaniya [Essays on sports pharmacology. Vol. 3. Pharmacoregulatory vectors]. Moscow, Saint Petersburg: Ajsing Publ., 2014. 356 p. (In Russian).
6. Sariev A.K., Abaimov D.A., Sejfulla R.D. Problema povysheniya biodostupnosti lekarstvennyh sredstv metodami nanofarmakologii: farmakokinetika liposomal’nyh preparatov [The problem of increasing the bioavailability of drugs by nanopharmacology methods: pharmacokinetics of liposomal drugs]. Experimental and Clinical Pharmacology. 2010:73(1):34–38. (In Russian). DOI: 10.30906/0869-2092-2010-73-11-34-38
7. Ujba V.V., Kotenko K.V., Korchazhkina N.B., Petrova N.B., Mikhajlova A.A. Primenenie muskusa kabargi v klinicheskoj praktike (metod. rekom.) [The use of musk musk deer in clinical practice (guidelines)]. Moscow, 2013. 18 p. (In Russian).
8. Karkischenko V.N., Dulya M.S., Khvostov D.V., Ageldinov R.A., Lyublinskiy S.L. Proteomic Analysis in the Identification of Active Components in the Preputial Gland Secretion of the Siberian Musk Deer. Biomedicine. 2019;15(1):35–47. DOI: 10.33647/2074- 5982-15-1-35-47
9. Li D., Chen B., Zhang L. The musk chemical composition and microbiota of Chinese forest musk deer males. Scientific Report. 2016.
Review
For citations:
Karkischenko V.N., Dulya M.S., Ageldinov R.A., Lyublinskiy S.L., Karkischenko N.N. A LIPOSOMAL COMPOSITION OF MUSC DEER PREPUTIAL GLAND EXTRACT AS A NEW AGENT OF ADAPTOGENIC ACTION. Journal Biomed. 2019;(4):34-45. (In Russ.) https://doi.org/10.33647/2074-5982-15-4-34-45
Views:
635
Email this article 
