CRYOPRESERVATION OF RAT BRAIN SLICES WITH DIFFERENT DURATION AND RESTORATION OF THEIR ELECTRICAL ACTIVITY
https://doi.org/10.33647/2074-5982-15-4-98-106
Abstract
Effects of cryopreservation of male Wistar rat brain slices with different duration (4, 8, 10, 23, and 90 days) on changes in their electrical activity was investigated. The amplitudes of AMPA- and NMDA-dependent glutamatergic ionotropic mechanisms were measured, as well as the action potential of the lateral olfactory tract (AP LOT) at a temperature of –20°C and subsequent warming to +37°C. After cryopreservation, these mechanisms were preserved and restored. We used the method of electrophysiological registration of the AMPA and NMDA potentials and the total AP LOT. After cryopreservation, the AMPA-dependent mechanisms and the activity of conductive LOT fi bers were restored to normothermal values. On the contrary, the recovery of NMDA-dependent mechanisms was incomplete and averaged 34% compared with the normothermal values. The results indicate that, after cryopreservation, the activity of basic ionotropic glutamatergic mechanisms in rat brain slices is restored.
About the Author
A. A. Mokrushin
Pavlov Institute of Physiology of the Russian Academy of Sciences
Russian Federation
Russian Federation
Dr. Sci. (Biol.),
199034, Saint Petersburg, Makarova embankment, 6
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Review
For citations:
Mokrushin A.A. CRYOPRESERVATION OF RAT BRAIN SLICES WITH DIFFERENT DURATION AND RESTORATION OF THEIR ELECTRICAL ACTIVITY. Journal Biomed. 2019;(4):98-106. (In Russ.) https://doi.org/10.33647/2074-5982-15-4-98-106
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