Effect of Resveratrol on the Development of Non-Alcoholic Fatty Liver Disease in Rats Fed on High-Fat High-Fructose Diet

Abstract

The beneficial effect of polyphenol resveratrol (RES) on liver diseases, including non-alcoholic fatty liver disease (NAFLD), has been reported in numerous studies. De novo lipogenesis in the liver is considered as one of the possible molecular mechanisms for the development of obesity and NAFLD. This work aimed to study the effect of RES on the development of NAFLD and to evaluate the expression of carbohydrate and lipid metabolic key enzymes genes in rats fed on a high-fat high-fructose diet (HFHFrD). For 10 weeks, the control group of Wistar male rats received a standard diet and water; three experimental groups were fed on HFHFrD. The rats in the 2nd and 3rd experimental groups received RES at a daily dose of 10 and 100 mg/kg body weight, respectively. A histological examination of the liver was carried out and the expression of carbohydrate (Khk, Gck, Pklr) and lipid (Acaca, Fasn, Scd) metabolizing enzymes and transcription factors (Mlxipl, Srebf1, Ppara) genes was studied. The consumption of HFHFrD led to the development of NAFLD, accompanied by an increase in Gck gene expression, a decrease in Pklr, Acaca, Fasn, Scd. RES in both doses had no effect on the development of NAFLD, as well as on the expression of the studied genes.