Effect of Chitosan on the Activity of Antimicrobial Agents in vitro
https://doi.org/10.33647/2074-5982-22-1-70-83
Abstract
The effect of chitosan on the efficacy of antimicrobial drugs was studied in vitro. It was established that the enterosorbents Lactofiltrum®, Chitosan Evalar®, and Enterosgel® exert no significant impact on the activity of antibiotics such as ciprofloxacin, amoxiclav, clarithromycin, and cefoperazone. Low-molecularweight crab chitosan (molecular weight (MW): 50 kDa; degree of deacetylation (DD): 85.0%) was found to enhance the efficacy of aminoglycosides (gentamicin and kanamycin), macrolides (clarithromycin), and fluoroquinolones (lomefloxacin and ofloxacin). Medium-molecular-weight crab chitosan (MW: 83.7 kDa; DD: 89.0%) showed a reduction in ofloxacin and lomefloxacin activity by 26.7% and 24.1%, respectively. High-molecular-weight fungal chitosan derived from Rhizopus oryzae F-814 (MW: 400 kDa; DD: 86.8%; ATCC 9363, NRRL 395, IMI 40564) exhibited a 25.4%–57.1% increase in ofloxacin efficacy, highlighting its potential for pharmacological applications. Preliminary sorption of antibiotics onto chitosan for two hours enhanced the antimicrobial effects observed under sequential administration of antibiotic solutions into assay
About the Authors
A. D. Beliaeva
Saint Petersburg Institute of Technology (Technical University)
Russian Federation
Russian Federation
Anna D. Beliaeva
190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26
G. G. Nianikova
Saint Petersburg Institute of Technology (Technical University)
Russian Federation
Russian Federation
Galina G. Nianikova, Cand. Sci. (Biol.), Assoc. Prof
190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26
A. R. Sakhipova
Saint Petersburg Institute of Technology (Technical University)
Russian Federation
Russian Federation
Alfiia R. Sakhipova
190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26
I. D. Beliaeva
Saint Petersburg Institute of Technology (Technical University)
Russian Federation
Russian Federation
Irina D. Beliaeva
190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26
O. Iu. Kharkova
Saint Petersburg Institute of Technology (Technical University)
Russian Federation
Russian Federation
Irina D. Beliaeva
190013, Russian Federation, Saint Petersburg, Moskovsky Ave., 26
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Review
For citations:
Beliaeva A.D., Nianikova G.G., Sakhipova A.R., Beliaeva I.D., Kharkova O.I. Effect of Chitosan on the Activity of Antimicrobial Agents in vitro. Journal Biomed. 2026;22(1):70-83. https://doi.org/10.33647/2074-5982-22-1-70-83
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