Features of the catalytic and kinetic properties of aldehyde dehydrogenase to the vital organs in experimental thermal injury

Abstract

The catalytic and kinetic properties of aldehyde dehydrogenase (ALDH) have been studied in the various organs of the experimental burns injury. The boiling water burn (20%) were caused to rats Wistar under ether anaesthesia, exposure – 3 sec. The animals were taken out of the experiment on the third, seventh and tenth day after the injury. Activity of aldehyde dehydrogenase and the concentration of the protein were determined in the homogenate of tissues (liver, kidney, heart and lungs). Of primary experimental data total kinetic curve of dependence of reaction product from the time were counted kinetic characteristics of ALDH (Kt, Vmax, Vmax/Kt). It was established that the thermal injury, the redistribution of overall aldehyde dehydrogenase activity between the liver, kidneys, heart and lungs is, in comparison with the control animals: when burning the highest total activity of ALDH is observed in the kidneys, the lowest - in the lungs (3, 7 days after the injury). Decrease of specific aldehyde dehydrogenase activity was revealed in the liver and the lungs (the most expressed on a 7 days after thermal damage), reduction in the rate of catalytic efficiency of ALDH and the increase in time of reaching . Vmax of enzymatic reaction. Increase of the specific activity of the enzyme was noted in the kidneys and the heart (the maximum of the 10-th day), due to the increase of the coefficient of catalytic efficiency and affinity of the enzyme to the substrate. The fall of general and specific activity of the aldehyde dehydrogenase in the liver to 3, 7 and 10 days after the burn indicates the greatest damage to the organ with thermal injury.