Studying of a differentiation of embryonic stem cells at a long kokultivirovaniye with adipocits
Abstract
In a mature monolayer adipocits, large spherical reflecting cages, did a scraper acellular paths of 1-5 mm of width. The flat spindle-shaped light-absorbing cages migrating from borders of paths, covered them with a monolayer in 8-10 days. The new monolayer is morphologically stable within several weeks after education and is sharply other than the old – its cage flat and dark, without reflecting inclusions (fatty drops). If to an adipotsitarny monolayer with the dug acellular paths to add mouse embryonic stem cells (MESK), the new monolayer which was formed to 6-7 day contains brilliant points, and by 15-20 day turns into a continuous brilliant carpet young адипоцитов, filled on the periphery of each cage tsirkulyarno the located small reflecting lipidic droplets. The question of a true or inductive differentiation, as to the reason of this phenomenon is discussed.
About the Authors
M. V. Kovina
Институт Биохимии им. Баха РАН,, Москва
Russian Federation
Russian Federation
L. . Zilberman
The University of Texas Health Science Center at Houston, Medical School, Department of Integrative Biology & Pharmacology
Russian Federation
Russian Federation
Yu. M. Hodarovich
Учреждение Российской академии наук Институт биооpганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН, Москва
Russian Federation
Russian Federation
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Review
For citations:
Kovina M.V., Zilberman L., Hodarovich Yu.M. Studying of a differentiation of embryonic stem cells at a long kokultivirovaniye with adipocits. Journal Biomed. 2013;1(4):107-113. (In Russ.)
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